Expression of two kinds of cytochrome P-450(11β) mrna in bovine adrenal cortex

Using pcP-450(11β)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559-568) as the probe, a different cDNA clone, pcP-450(11β)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11β)-3 was highly homologous but not identical with that of pcP-450(11β)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11β)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11β) mRNA were expressed in individual animals and that a least two kinds of P-450(11β) genes exist in the bovine genome.