TY - JOUR
T1 - Expression of substance P receptor in the substantia nigra
AU - Futami, T.
AU - Hatanaka, Y.
AU - Matsushita, K.
AU - Furuya, S.
N1 - Funding Information:
The authors express their sincere thanks to colleagues in the Riken Institute: Drs. E.G. Jones and T. Hashikawa for valuable discussion and critical reading of the manuscript; Dr Y. Hirabayashi for his kind and helpful arrangement; and Mr. H. Kishida for his technical assistance. This study was supported by the Frontier Research Program, RIKEN, and by a grant from the Japanese Ministry of Education, Science and Culture for Scientific Research.
PY - 1998/3/1
Y1 - 1998/3/1
N2 - Since the substantia nigra receives abundant substance P innervations but lacks clear evidences about a presence of substance P receptors, expressions for mRNA and protein of substance P receptors were investigated in the rat to resolve this mismatch. Expression levels of substance P receptors mRNA in the substantia nigra pars compacta and reticulata were 37.7 and 24.1% of those in the striatum, respectively, by reverse transcription-polymerase chain reaction (RT-PCR). Substance P receptors mRNA was found in dopamine neurons of the substantia nigra pars compacta by single cell RT-PCR. Ca. 90% of dopamine neurons in the substantia nigra pars compacta were immunoreactive to anti-substance P receptor antibody in the colchicine treated rats. These are the first direct evidence for the existence of substance P receptors in dopamine neurons of the substantia nigra pars compacta.
AB - Since the substantia nigra receives abundant substance P innervations but lacks clear evidences about a presence of substance P receptors, expressions for mRNA and protein of substance P receptors were investigated in the rat to resolve this mismatch. Expression levels of substance P receptors mRNA in the substantia nigra pars compacta and reticulata were 37.7 and 24.1% of those in the striatum, respectively, by reverse transcription-polymerase chain reaction (RT-PCR). Substance P receptors mRNA was found in dopamine neurons of the substantia nigra pars compacta by single cell RT-PCR. Ca. 90% of dopamine neurons in the substantia nigra pars compacta were immunoreactive to anti-substance P receptor antibody in the colchicine treated rats. These are the first direct evidence for the existence of substance P receptors in dopamine neurons of the substantia nigra pars compacta.
UR - https://www.scopus.com/pages/publications/0032031799
UR - https://www.scopus.com/pages/publications/0032031799#tab=citedBy
U2 - 10.1016/S0169-328X(97)00307-0
DO - 10.1016/S0169-328X(97)00307-0
M3 - Article
C2 - 9555006
AN - SCOPUS:0032031799
SN - 0169-328X
VL - 54
SP - 183
EP - 198
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 2
ER -