Expression of nicotiana glutinosa ribonucleases in escherichia coli

Madoka Hino, Shin Kawano, Makoto Kimura

研究成果: ジャーナルへの寄稿学術誌査読

5 被引用数 (Scopus)

抄録

We previously isolated from Nicotiana glutinosa leaves three distinct cDNA clones, NGR1, NGR2, and NGR3, encoding a wound-inducible RNase NW, and putative RNases NGR2 and NGR3, respectively. In this study, we produced RNases NW and NGR3 in Escherichia coli and purified them to homogeneity. RNase NGR3 had non-absolute specificity toward polynucleotides, although RNase NW preferentially cleaved polyinosinic acid (Poly I). Both RNases NW and NGR3 were more active toward diribonucleoside monophosphates ApG, CpU, and GpU. Furthermore, kinetic parameters for RNase NW (Km, 0.778 mM and kcat, 1938 min−1) and RNase NGR3 (Km, 0.548 mM and kcat, 408 min−1) were calculated using GpU as a substrate.

本文言語英語
ページ(範囲)910-912
ページ数3
ジャーナルBioscience, Biotechnology and Biochemistry
66
4
DOI
出版ステータス出版済み - 2002

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

フィンガープリント

「Expression of nicotiana glutinosa ribonucleases in escherichia coli」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル