Evolutionary screening of collagen-like peptides that nucleate hydroxyapatite crystals

Woo Jae Chung, Ki Young Kwon, Jie Song, Seung Wuk Lee

研究成果: ジャーナルへの寄稿学術誌査読

69 被引用数 (Scopus)

抄録

The biogenesis of inorganic/organic composite materials such as bone typically involves the process of templated mineralization. Biomimetic synthesis of bone-like materials therefore requires the development of organic scaffolds that mediate mineralization of hydroxyapatite (HAP), the major inorganic component of bone. Using phage display, we identified a 12-residue peptide that bound to single-crystal HAP and templated the nucleation and growth of crystalline HAP mineral in a sequence- and composition-dependent manner. The sequence responsible for the mineralizing activity resembled the tripeptide repeat (Gly-Pro-Hyp) of type I collagen, a major component of bone extracellular matrix. Using a panel of synthetic peptides, we defined the structural features required for mineralizing activity. The results support a model for the cooperative noncovalent interaction of the peptide with HAP and suggest that native collagen may have a mineral-templating function in vivo. We expect this short HAP-binding peptide to be useful in the synthesis of three-dimensional bone-like materials.

本文言語英語
ページ(範囲)7620-7628
ページ数9
ジャーナルLangmuir
27
12
DOI
出版ステータス出版済み - 6月 21 2011
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 材料科学一般
  • 凝縮系物理学
  • 表面および界面
  • 分光学
  • 電気化学

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