Establishment of rat embryonic stem cells and making of chimera rats

Shinobu Ueda, Masaki Kawamata, Takumi Teratani, Taku Shimizu, Yoshitaka Tamai, Hiromasa Ogawa, Katsuyuki Hayashi, Hiroyuki Tsuda, Takahiro Ochiya

研究成果: ジャーナルへの寄稿学術誌査読

54 被引用数 (Scopus)

抄録

The rat is reference animal model for physiological studies and for the analysis of multigenic human disease such as hypertension, disease, neurological disorders, and cancer. The rats have long been used in extensive chemical carcinogenesis studies. Thus, the rat embryonic stem (rES) cell is an important resource for the study of disease models. Attempts to derive ES cells from various mammals, inlcuding the rat, have not succeeded. Here we have established two independent rES cells from Wister rat blastocytes that have undifferentiated characters such as Nanog and Oct3/4 genes expression and they have stage-specific embryonic antigen (SSEA) -1, -3, -4, and TRA-1-81 expression. The cells were successfully cultured in an undifferentiated state and can be possible over 18 passages with maintaining more than 40% of normal karyotype. Teh pluripotent was confirmed by the differentiation into derivatives of the endoderm, mesoderm, and ectoderm. Most importantly, the rES cells are capable of producing chimera rats. Therefore, we established pluripotent rES cell lines that are widely used to produce genetically modified experimental rat for study of human diseases.

本文言語英語
論文番号e2800
ジャーナルPloS one
3
7
DOI
出版ステータス出版済み - 7月 30 2008
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学一般
  • 農業および生物科学一般
  • 一般

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