Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: In vivo differentiation into lymphocytes and potential for germ line transmission

Seiho Nagafuchi; Hitoshi Katsuta; Kazuhiko Kogawa; Tomoyuki Akashi; Shiori Kondo; Yumiko Sakai; Tadasuke Tsukiyama; Daisuke Kitamura; Yoshiyuki Niho; Takeshi Watanabe

doi:10.1016/S0014-5793(99)00801-7

Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: In vivo differentiation into lymphocytes and potential for germ line transmission

Seiho Nagafuchi
, Hitoshi Katsuta
, Kazuhiko Kogawa
, Tomoyuki Akashi
, Shiori Kondo
, Yumiko Sakai
, Tadasuke Tsukiyama
, Daisuke Kitamura
, Yoshiyuki Niho
, Takeshi Watanabe

検査技術科学

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

36 被引用数 (Scopus)

抄録

A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(β)(g7) haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established. Copyright (C) 1999 Federation of European Biochemical Societies.

本文言語	英語
ページ（範囲）	101-104
ページ数	4
ジャーナル	FEBS Letters
巻	455
号	1-2
DOI	https://doi.org/10.1016/S0014-5793(99)00801-7
出版ステータス	出版済み - 7月 16 1999

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!!!All Science Journal Classification (ASJC) codes

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/S0014-5793(99)00801-7

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「Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: In vivo differentiation into lymphocytes and potential for germ line transmission」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

Nagafuchi, S., Katsuta, H., Kogawa, K., Akashi, T., Kondo, S., Sakai, Y., Tsukiyama, T., Kitamura, D., Niho, Y., & Watanabe, T. (1999). Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: In vivo differentiation into lymphocytes and potential for germ line transmission. FEBS Letters, 455(1-2), 101-104. https://doi.org/10.1016/S0014-5793(99)00801-7

Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: In vivo differentiation into lymphocytes and potential for germ line transmission. / Nagafuchi, Seiho; Katsuta, Hitoshi; Kogawa, Kazuhiko その他.
In: FEBS Letters, Vol. 455, No. 1-2, 16.07.1999, p. 101-104.

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

Nagafuchi, S, Katsuta, H, Kogawa, K, Akashi, T, Kondo, S, Sakai, Y, Tsukiyama, T, Kitamura, D, Niho, Y & Watanabe, T 1999, 'Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: In vivo differentiation into lymphocytes and potential for germ line transmission', FEBS Letters, vol. 455, no. 1-2, pp. 101-104. https://doi.org/10.1016/S0014-5793(99)00801-7

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title = "Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: In vivo differentiation into lymphocytes and potential for germ line transmission",

abstract = "A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(β)(g7) haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Diabetes, Embryonic stem cell, Germ line transmission, Insulitis, Non-obese diabetic mouse",

author = "Seiho Nagafuchi and Hitoshi Katsuta and Kazuhiko Kogawa and Tomoyuki Akashi and Shiori Kondo and Yumiko Sakai and Tadasuke Tsukiyama and Daisuke Kitamura and Yoshiyuki Niho and Takeshi Watanabe",

note = "Funding Information: The authors thank Yukari Yamada, Laboratory of Embryonic and Genetic Engineering, Medical Institute of Bioregulation, Kyushu University, for her excellent technical assistance. We also express our sincere thanks to Dr. Peter D. Burrows for his critical reading of the manuscript. This work is supported in part by a grant from Kyushu University Interdisciplinary Programs in Education and Projects in Research Development. ",

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AU - Kogawa, Kazuhiko

AU - Akashi, Tomoyuki

AU - Kondo, Shiori

AU - Sakai, Yumiko

AU - Tsukiyama, Tadasuke

AU - Kitamura, Daisuke

AU - Niho, Yoshiyuki

AU - Watanabe, Takeshi

N1 - Funding Information: The authors thank Yukari Yamada, Laboratory of Embryonic and Genetic Engineering, Medical Institute of Bioregulation, Kyushu University, for her excellent technical assistance. We also express our sincere thanks to Dr. Peter D. Burrows for his critical reading of the manuscript. This work is supported in part by a grant from Kyushu University Interdisciplinary Programs in Education and Projects in Research Development.

PY - 1999/7/16

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AB - A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(β)(g7) haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Diabetes

KW - Embryonic stem cell

KW - Germ line transmission

KW - Insulitis

KW - Non-obese diabetic mouse

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