Establishment of ameloblastoma cell line, AM-1

Hidemitsu Harada, Takeshi Mitsuyasu, Norifumi Nakamura, Yoshinori Higuchi, Kuniaki Toyoshima, Akiyoshi Taniguchi, Shigeru Yasumoto

研究成果: ジャーナルへの寄稿学術誌査読

54 被引用数 (Scopus)


Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.

ジャーナルJournal of Oral Pathology and Medicine
出版ステータス出版済み - 5月 1998

!!!All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 口腔外科
  • 耳鼻咽喉科学
  • 癌研究
  • 歯周病学


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