Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies

Osamu Morinaga, Takuhiro Uto, Seiichi Sakamoto, Hiroyuki Tanaka, Yukihiro Shoyama

研究成果: ジャーナルへの寄稿学術誌査読

11 被引用数 (Scopus)

抄録

Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.

本文言語英語
ページ(範囲)28-31
ページ数4
ジャーナルFitoterapia
80
1
DOI
出版ステータス出版済み - 1月 2009

!!!All Science Journal Classification (ASJC) codes

  • 薬理学
  • 創薬

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