Enhancement of the antisense effect of polysaccharide-polynucleotide complexes by preventing the antisense oligonucleotide from binding to proteins in the culture medium

Masami Mizu, Kazuya Koumoto, Takahisa Anada, Ryouji Karinaga, Taro Kimura, Takeshi Nagasaki, Seiji Shinkai, Kazuo Sakurai

研究成果: ジャーナルへの寄稿学術誌査読

29 被引用数 (Scopus)

抄録

Schizophyllan is a β-(1 → 3)-D-glucan existing as a triple helix in water and as a single chain in dimethyl sulfoxide (DMSO), respectively. As we already reported, when a homo-phosphodiester-polynucleotide is added to the schizophyllan/DMSO solution and, subsequently, DMSO is exchanged for water, the single chain of schizophyllan (s-SPG) forms a complex with the polynucleotide. In this paper, we report that phosphorothioate oligonucleotides can form a complex with s-SPG in the same manner as phosphodiester oligonucleotides. We carried out an in vitro antisense assay combining melanoma cell lines and a phosphorothioate antisense oligonucleotide (AS ODN) to depress c-myb mRNA. We found that the AS ODN bound to the complex reduces cell growth more efficiently than that of naked AS ODN by preventing the AS ODN from binding to albumin in the culture medium and being hydrolyzed.

本文言語英語
ページ(範囲)1101-1110
ページ数10
ジャーナルBulletin of the Chemical Society of Japan
77
6
DOI
出版ステータス出版済み - 2004
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 化学一般

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