TY - JOUR
T1 - Enhanced NRT1.1/NPF6.3 expression in shoots improves growth under nitrogen deficiency stress in Arabidopsis
AU - Sakuraba, Yasuhito
AU - Chaganzhana,
AU - Mabuchi, Atsushi
AU - Iba, Koh
AU - Yanagisawa, Shuichi
N1 - Funding Information:
We thank the ABRC for providing seeds of 51 Arabidopsis accessions and the chl1-5 mutant. We also thank Sachiyo Nagumo for providing assistance with the experiments performed in this study. This work was supported in part by the Core Research for Evolutional Science and Technology, Japan Science and Technology Agency (JPMJCR 15O5 to S.Y.), the Kyushu University Qdai-jump Research Program (grant nos. 01325, 02306 to A.M.) and by the Japan Society for the Promotion of Science KAKENHI (18H03940 to S.Y., 20H03279 to K.I. and 17H05024 to Y.S.).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Identification of genes and their alleles capable of improving plant growth under low nitrogen (N) conditions is key for developing sustainable agriculture. Here, we show that a genome-wide association study using Arabidopsis thaliana accessions suggested an association between different magnitudes of N deficiency responses and diversity in NRT1.1/NPF6.3 that encodes a dual-affinity nitrate transporter involved in nitrate uptake by roots. Various analyses using accessions exhibiting reduced N deficiency responses revealed that enhanced NRT1.1 expression in shoots rather than in roots is responsible for better growth of Arabidopsis seedlings under N deficient conditions. Furthermore, polymorphisms that increased NRT1.1 promoter activity were identified in the NRT1.1 promoter sequences of the accessions analyzed. Hence, our data indicated that polymorphism-dependent activation of the NRT1.1 promoter in shoots could serve as a tool in molecular breeding programs for improving plant growth in low N environments.
AB - Identification of genes and their alleles capable of improving plant growth under low nitrogen (N) conditions is key for developing sustainable agriculture. Here, we show that a genome-wide association study using Arabidopsis thaliana accessions suggested an association between different magnitudes of N deficiency responses and diversity in NRT1.1/NPF6.3 that encodes a dual-affinity nitrate transporter involved in nitrate uptake by roots. Various analyses using accessions exhibiting reduced N deficiency responses revealed that enhanced NRT1.1 expression in shoots rather than in roots is responsible for better growth of Arabidopsis seedlings under N deficient conditions. Furthermore, polymorphisms that increased NRT1.1 promoter activity were identified in the NRT1.1 promoter sequences of the accessions analyzed. Hence, our data indicated that polymorphism-dependent activation of the NRT1.1 promoter in shoots could serve as a tool in molecular breeding programs for improving plant growth in low N environments.
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U2 - 10.1038/s42003-021-01775-1
DO - 10.1038/s42003-021-01775-1
M3 - Article
C2 - 33637855
AN - SCOPUS:85101752156
SN - 2399-3642
VL - 4
JO - Communications Biology
JF - Communications Biology
IS - 1
M1 - 256
ER -