TY - JOUR
T1 - Enhanced erythroid cell differentiation in hypoxic condition is in part contributed by miR-210
AU - Sarakul, Orawan
AU - Vattanaviboon, Phantip
AU - Tanaka, Yuka
AU - Fucharoen, Suthat
AU - Abe, Yasunobu
AU - Svasti, Saovaros
AU - Umemura, Tsukuru
N1 - Funding Information:
This study was supported in part by Office of the Higher Education Commission and Mahidol University under the National Research University Initiative , the Thailand Research Fund (TRF) , the Research Chair Grant , the National Science and Technology Development Agency, Thailand (NSTDA) and the Japan Society for the Promotion of Science (JSPS) . OS was supported by the Strategic Scholarship Fellowship Frontier Research Networks , Office of the Higher Education Commission and Mahidol University.
PY - 2013/8
Y1 - 2013/8
N2 - Erythropoiesis, a process of erythroid production, is controlled by several factors including oxygen level. In this study, the effect of oxygen tension on erythropoiesis was investigated in K562 erythroleukemic cell line and erythroid progenitor cells derived from normal and β-thalassemia/hemoglobin (Hb) E individuals. The enhanced erythroid differentiation specific markers including increased levels of α-, β- and γ-globin gene expressions, numbers of HbF positive cells and the presence of glycophorin A surface marker were observed during cell culture under hypoxic atmosphere. The result also showed that miR-210, one of the hypoxia-induced miRNAs, was up-regulated in K562 and β-thalassemia/HbE progenitor cells cultured under hypoxic condition. Inhibition of miR-210 expression leads to reduction of the globin gene expression and delayed maturation in K562 and erythroid progenitor cells. This indicated that miR-210 contributes to hypoxia-induced erythroid differentiation in both K562 cells and β-thalassemia/HbE erythroid progenitor cells.
AB - Erythropoiesis, a process of erythroid production, is controlled by several factors including oxygen level. In this study, the effect of oxygen tension on erythropoiesis was investigated in K562 erythroleukemic cell line and erythroid progenitor cells derived from normal and β-thalassemia/hemoglobin (Hb) E individuals. The enhanced erythroid differentiation specific markers including increased levels of α-, β- and γ-globin gene expressions, numbers of HbF positive cells and the presence of glycophorin A surface marker were observed during cell culture under hypoxic atmosphere. The result also showed that miR-210, one of the hypoxia-induced miRNAs, was up-regulated in K562 and β-thalassemia/HbE progenitor cells cultured under hypoxic condition. Inhibition of miR-210 expression leads to reduction of the globin gene expression and delayed maturation in K562 and erythroid progenitor cells. This indicated that miR-210 contributes to hypoxia-induced erythroid differentiation in both K562 cells and β-thalassemia/HbE erythroid progenitor cells.
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U2 - 10.1016/j.bcmd.2013.03.005
DO - 10.1016/j.bcmd.2013.03.005
M3 - Article
C2 - 23623309
AN - SCOPUS:84878398511
SN - 1079-9796
VL - 51
SP - 98
EP - 103
JO - Blood Cells, Molecules, and Diseases
JF - Blood Cells, Molecules, and Diseases
IS - 2
ER -