Engineered protein-tag for rapid live-cell fluorogenic visualization of proteins by anionic probes

Jingchi Gao, Yuichiro Hori, Miyako Nishiura, Mathieu Bordy, Jens Hasserodt, Kazuya Kikuchi

研究成果: ジャーナルへの寄稿総説査読

5 被引用数 (Scopus)

抄録

Live-cell protein labeling using a protein tag and a fluorescent probe is a powerful approach for studying protein localization and dynamics. Herein, we engineered a protein tag, PYP-tag, to image membrane proteins with high contrast. Mutations of charged amino acids on the surface of PYP-tag led to the enhancement of labeling kinetics and brightness. Furthermore, the modification of the surface charge did not affect its expression on the cell surface, and we succeeded in applying this labeling technique to high-contrast imaging of translocation of glucose transporter 4.

本文言語英語
ページ(範囲)232-235
ページ数4
ジャーナルChemistry Letters
49
3
DOI
出版ステータス出版済み - 2020
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 化学一般

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