TY - JOUR
T1 - Engineered Nanogel Particles Enhance the Photoautotrophic Biosynthesis of Polyhydroxyalkanoate in Marine Photosynthetic Bacteria
AU - Srisawat, Pisanee
AU - Higuchi-Takeuchi, Mieko
AU - Honda, Ryutaro
AU - Shirai, Tomokazu
AU - Kondo, Akihiko
AU - Hoshino, Yu
AU - Numata, Keiji
N1 - Funding Information:
This study was funded by the ImPACT Program of the Council for Science, Technology and Innovation (Cabinet office, Government of Japan), JST ERATO (grant no JPMJER1602), Grant-in-Aid for Transformative Research Areas (B), and Material DX. We thank Dr. Choon Pin Foong for the helpful discussion and technical support for CLSM analysis. We also thank Dr. Seiya Fujita for the helpful discussion and technical support for surface zeta potential measurement.
Publisher Copyright:
© 2022 The Authors. Published by American Chemical Society.
PY - 2022/4/4
Y1 - 2022/4/4
N2 - Improving polyhydroxyalkanoate (PHA, a biodegradable plastic) production under photoautotrophic cultivation is challenging for sustainable bioproduction. In this study, we demonstrated the use of engineered nanogel particles to enhance PHA accumulation in the marine photosynthetic bacterium Rhodovulum sulfidophilum under photoautotrophic culture. We screened the effect of 13 engineered nanogel particles on the cell growth and PHA accumulation of R. sulfidophilum. The addition of anionic nanogel particles significantly enhanced PHA accumulation in R. sulfidophilum up to 157-fold compared to that without nanogel particles. By performing 13C tracer experiments and gas chromatography-mass spectrometry analysis, we confirmed that HCO3- was assimilated throughout the central carbon metabolism and that the accumulated PHA was indeed incorporated from HCO3-. Our results indicate successful PHA production with the supplementation of engineered nanogel particles under photoautotrophic cultivation in R. sulfidophilum. Furthermore, the strategy of using engineered nanoparticles demonstrated in this study may be applicable to other microbial cell factories to produce other commodity metabolites.
AB - Improving polyhydroxyalkanoate (PHA, a biodegradable plastic) production under photoautotrophic cultivation is challenging for sustainable bioproduction. In this study, we demonstrated the use of engineered nanogel particles to enhance PHA accumulation in the marine photosynthetic bacterium Rhodovulum sulfidophilum under photoautotrophic culture. We screened the effect of 13 engineered nanogel particles on the cell growth and PHA accumulation of R. sulfidophilum. The addition of anionic nanogel particles significantly enhanced PHA accumulation in R. sulfidophilum up to 157-fold compared to that without nanogel particles. By performing 13C tracer experiments and gas chromatography-mass spectrometry analysis, we confirmed that HCO3- was assimilated throughout the central carbon metabolism and that the accumulated PHA was indeed incorporated from HCO3-. Our results indicate successful PHA production with the supplementation of engineered nanogel particles under photoautotrophic cultivation in R. sulfidophilum. Furthermore, the strategy of using engineered nanoparticles demonstrated in this study may be applicable to other microbial cell factories to produce other commodity metabolites.
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U2 - 10.1021/acssuschemeng.1c07252
DO - 10.1021/acssuschemeng.1c07252
M3 - Article
AN - SCOPUS:85127909519
SN - 2168-0485
VL - 10
SP - 4133
EP - 4142
JO - ACS Sustainable Chemistry and Engineering
JF - ACS Sustainable Chemistry and Engineering
IS - 13
ER -
