Endogenous ROS production in early differentiation state suppresses endoderm differentiation via transient FOXC1 expression

Sugako Oka, Teruhisa Tsuzuki, Masumi Hidaka, Mizuki Ohno, Yoshimichi Nakatsu, Mutsuo Sekiguchi

研究成果: ジャーナルへの寄稿学術誌査読

3 被引用数 (Scopus)

抄録

Oxidative stress plays a pivotal role in the differentiation and proliferation of cells and programmed cell death. However, studies on the role of oxidative stress in differentiation have mainly employed the detection of reactive oxygen species (ROS) during differentiation or generated by ROS inducers. Therefore, it is difficult to clarify the significance of endogenous ROS production in the differentiation of human cells. We developed a system to control the intracellular level of ROS in the initial stage of differentiation in human iPS cells. By introducing a specific substitution (I69E) into the SDHC protein, a component of the mitochondrial respiratory chain complex, the endogenous ROS level increased. This caused impaired endoderm differentiation of iPS cells, and this impairment was reversed by overproduction of mitochondrial-targeted catalase, an anti-oxidant enzyme. Expression of tumor-related FOXC1 transcription factor increased transiently as early as 4 h after ROS-overproduction in the initial stage of differentiation. Knockdown of FOXC1 markedly improved impaired endoderm differentiation, suggesting that endogenous ROS production in the early differentiation state suppresses endoderm differentiation via transient FOXC1 expression.

本文言語英語
論文番号150
ジャーナルCell Death Discovery
8
1
DOI
出版ステータス出版済み - 12月 2022

!!!All Science Journal Classification (ASJC) codes

  • 免疫学
  • 細胞および分子神経科学
  • 細胞生物学
  • 癌研究

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