Effects of volatile and intravenous anesthetics on the uptake of GABA, glutamate and dopamine by their transporters heterologously expressed in COS cells and in rat brain synaptosomes

Mitsutaka Sugimura, Shigeo Kitayama, Katsuya Morita, Masahiro Irifune, Tohru Takarada, Michio Kawahara, Toshihiro Dohi

研究成果: ジャーナルへの寄稿学術誌査読

31 被引用数 (Scopus)

抄録

Although the neurotransmitter uptake system is considered a possible target for the presynaptic action of anesthetic agents, observations are inconsistent concerning effects on the transporter and their clinical relevance. The present study examined the effects of volatile and intravenous anesthetics on the uptake of GABA, glutamate and dopamine in COS cells heterologously expressing the transporters for these neurotransmitters and in the rat brain synaptosomes. Halothane and isoflurane, but not thiamylal or thiopental, significantly inhibited uptake by COS cell systems of GABA, dopamine and glutamic acid in a concentration-dependent manner within clinically relevant ranges for anesthesia induced by these agents. Similarly, in synaptosomes halothane and isoflurane but not thiopental significantly suppressed the uptake of GABA and glutamic acid, respectively. These results do not support the hypothesis that volatile and intravenous anesthetics exert their action via specific inhibition of GABA uptake to enhance inhibitory GABAergic neuronal activity. Rather, they suggest that presynaptic uptake systems for various neurotransmitters including GABA may be the molecular targets for volatile anesthetic agents.

本文言語英語
ページ(範囲)69-76
ページ数8
ジャーナルToxicology Letters
123
1
DOI
出版ステータス出版済み - 8月 6 2001
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 毒物学

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