Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor

Masamichi Kamihira; Hitoshi Yoshida; Shinji Iijima; Takeshi Kobayashi

doi:10.1016/0922-338X(90)90113-B

Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor

Masamichi Kamihira, Hitoshi Yoshida, Shinji Iijima, Takeshi Kobayashi

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

9 被引用数 (Scopus)

抄録

Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.

本文言語	英語
ページ（範囲）	311-312
ページ数	2
ジャーナル	Journal of Fermentation and Bioengineering
巻	69
号	5
DOI	https://doi.org/10.1016/0922-338X(90)90113-B
出版ステータス	出版済み - 1990
外部発表	はい

!!!All Science Journal Classification (ASJC) codes

バイオテクノロジー
応用微生物学とバイオテクノロジー

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10.1016/0922-338X(90)90113-B

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引用スタイル

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title = "Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor",

abstract = "Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.",

author = "Masamichi Kamihira and Hitoshi Yoshida and Shinji Iijima and Takeshi Kobayashi",

year = "1990",

doi = "10.1016/0922-338X(90)90113-B",

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T1 - Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor

AU - Kamihira, Masamichi

AU - Yoshida, Hitoshi

AU - Iijima, Shinji

AU - Kobayashi, Takeshi

PY - 1990

Y1 - 1990

N2 - Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.

AB - Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.

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U2 - 10.1016/0922-338X(90)90113-B

DO - 10.1016/0922-338X(90)90113-B

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IS - 5

ER -

Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor

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!!!All Science Journal Classification (ASJC) codes

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