Effects of hemicelluloses on dehydrogenative polymerization of monolignols with cationic cell wall-bound peroxidase

Yan Lyu, Shiori Suzuki, Hiroki Nagano, Kengo Shigetomi, Yutaka Tamai, Yuji Tsutsumi, Yasumitsu Uraki

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

To elucidate the influence of polysaccharides on hardwood lignification, dehydrogenative polymerization of monolignols, coniferyl alcohol (CA) and sinapyl alcohol (SA), was attempted with recombinant cationic cell wall-bound peroxidase (rCWPO-C) and horseradish peroxidase (HRP) in measurement cells of a quartz crystal microbalance with dissipation (QCM-D). Hardwood cellulose nanofibers were anchored; hemicelluloses, xylan, partially acetylated xylan (AcXY), galactoglucomannan, and xyloglucan, and the enzymes were subsequently adsorbed onto the QCM-D sensor surface, enabling fabrication of artificial polysaccharide matrices. The largest amount of rCWPO-C is found to be adsorbed onto AcXY among all the polysaccharides, which affords the largest amount and size of spherical dehydrogenation polymers (DHPs) from both CA and SA. In contrast, no DHP and a small amount of DHPs are formed from SA and CA, respectively, by HRP catalysis in all of the polysaccharide matrices. This study demonstrates important functions of a real tree-derived peroxidase, rCWPO-C, and AcXY for hardwood lignification.

本文言語英語
論文番号120305
ジャーナルCarbohydrate Polymers
301
DOI
出版ステータス出版済み - 2月 1 2023

!!!All Science Journal Classification (ASJC) codes

  • 材料化学
  • ポリマーおよびプラスチック
  • 有機化学

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