TY - JOUR
T1 - Effect of poly(L-lactide) surface topography on the morphology of in vitro cultured human articular chondrocytes
AU - Costa Martínez, E.
AU - Escobar Ivirico, J. L.
AU - Muñoz Criado, I.
AU - Gómez Ribelles, J. L.
AU - Monleón Pradas, M.
AU - Salmerón Sánchez, M.
N1 - Funding Information:
Acknowledgements The authors are grateful to Kadriye Tuzlako-glu for providing the PLLA used in this work, the support of CICYT through the MAT2004–04980–C02–01 Project, and the support to their research group by the Generalitat Valenciana through the project GRUPOS03/018. AFM and SEM was conducted by the authors in the Microscopy Service of the Universidad Politécnica de Valencia, whose advice was greatly appreciated.
PY - 2007/8
Y1 - 2007/8
N2 - Human articular chondrocytes were cultured in vitro on poly(L-lactic) acid, PLLA, substrates. Influence of the surface topography on cell morphology was found. Different surface microtopographies were obtained on PLLA by crystallizing at 120°C after nucleation treatments that include isothermal stages at temperatures just below (55°C) and just above (75°C) the glass transition temperature (T g = 65°C). Isothermal crystallization from the melt gave rise to big spherulites (approx. 50 μm diameter) with approx. 1 μm depth. Crystallization after nucleation treatments results in smaller (approx. 5 μm)-difficult to distinguish-spherulites. Cell viability was excellent and not affected by the surface roughness. Cell population on the nucleated samples resembles the result of culture on the reference tissue culture polystyrene (TCPS). However, cells cultured on big spherulites (PLLA isothermally crystallized without nucleation treatment) show a peculiar morphology, with a more isolated disposition and growth oriented in a characteristic direction.
AB - Human articular chondrocytes were cultured in vitro on poly(L-lactic) acid, PLLA, substrates. Influence of the surface topography on cell morphology was found. Different surface microtopographies were obtained on PLLA by crystallizing at 120°C after nucleation treatments that include isothermal stages at temperatures just below (55°C) and just above (75°C) the glass transition temperature (T g = 65°C). Isothermal crystallization from the melt gave rise to big spherulites (approx. 50 μm diameter) with approx. 1 μm depth. Crystallization after nucleation treatments results in smaller (approx. 5 μm)-difficult to distinguish-spherulites. Cell viability was excellent and not affected by the surface roughness. Cell population on the nucleated samples resembles the result of culture on the reference tissue culture polystyrene (TCPS). However, cells cultured on big spherulites (PLLA isothermally crystallized without nucleation treatment) show a peculiar morphology, with a more isolated disposition and growth oriented in a characteristic direction.
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U2 - 10.1007/s10856-007-3038-1
DO - 10.1007/s10856-007-3038-1
M3 - Article
C2 - 17483895
AN - SCOPUS:34447120183
SN - 0957-4530
VL - 18
SP - 1627
EP - 1632
JO - Journal of Materials Science: Materials in Medicine
JF - Journal of Materials Science: Materials in Medicine
IS - 8
ER -