Effect of organic solvents on extraction behaviour of cytochrome c using reversed micellar solutions

Tsutomu Ono, Takayuki Miyazaki, Masahiro Goto, Fumiyuki Nakashio

    研究成果: ジャーナルへの寄稿学術誌査読

    8 被引用数 (Scopus)

    抄録

    The effect of organic solvents on the extraction behaviour of cytochrome c has been investigated with reversed micellar solutions. An aliphatic solvent such as hexane, octane or isooctane was suitable for protein extraction operations using the reversed micelles. Organic solvents having a different hydrophobicity show significantly different behaviour in protein extraction, where extraction efficiency appears to be governed by the nature of the interfacial complex that is formed by surfactants and proteins. Solvent effects on the protein extraction are more dominant in the AOT system rather than that in the DOLPA system. Addition of alcohol appears to decrease the activity of proteins in the back-extraction step, although it is effective for recovering proteins from the reversed micellar solutions.

    本文言語英語
    ページ(範囲)1-9
    ページ数9
    ジャーナルSolvent Extraction Research and Development, Japan
    1996
    3
    出版ステータス出版済み - 1996

    !!!All Science Journal Classification (ASJC) codes

    • 化学一般
    • 化学工学一般
    • ろ過および分離

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