TY - JOUR
T1 - Effect of Alkynyl Group on Reactivity in Photoaffinity Labeling with 2-Thienyl-Substituted α-Ketoamide
AU - Moriyama, Takahiro
AU - Mizukami, Daiki
AU - Yoritate, Makoto
AU - Usui, Kazuteru
AU - Takahashi, Daisuke
AU - Ota, Eisuke
AU - Sodeoka, Mikiko
AU - Ueda, Tadashi
AU - Karasawa, Satoru
AU - Hirai, Go
N1 - Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2022/2/19
Y1 - 2022/2/19
N2 - Minimalist photo-reactive probes, which consist of a photo-reactive group and a tag for detection of target proteins, are useful tools in chemical biology. Although several diazirine-based and aryl azide-based minimalist probes are available, no keto-based minimalist probe has yet been reported. Here we describe minimalist probes based on a 2-thienyl-substituted α-ketoamide bearing an alkyne group on the thiophene ring. The 3-alkyne probe showed the highest photo-affinity labeling efficiency.
AB - Minimalist photo-reactive probes, which consist of a photo-reactive group and a tag for detection of target proteins, are useful tools in chemical biology. Although several diazirine-based and aryl azide-based minimalist probes are available, no keto-based minimalist probe has yet been reported. Here we describe minimalist probes based on a 2-thienyl-substituted α-ketoamide bearing an alkyne group on the thiophene ring. The 3-alkyne probe showed the highest photo-affinity labeling efficiency.
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U2 - 10.1002/chem.202103925
DO - 10.1002/chem.202103925
M3 - Article
C2 - 35023607
AN - SCOPUS:85123892376
SN - 0947-6539
VL - 28
JO - Chemistry - A European Journal
JF - Chemistry - A European Journal
IS - 11
M1 - e202103925
ER -