TY - JOUR
T1 - DNA sensors using a ferrocene-oligonucleotide conjugate
AU - Nakayama, Masamichi
AU - Ihara, Toshihiro
AU - Nakano, Koji
AU - Maeda, Mizuo
N1 - Funding Information:
This work was partially supported by a Grant-in-Aid for Encouragement of Young Scientists (No. 08750941) and by a Grant-in-Aid for Scientific Research (B) (No. 09555266) from The Ministry of Education, Culture, Sports, Science, and Technology (MEXT). The authors are also grateful to The Sagawa Foundation for Promotion of Frontier Science (to T.I.) and to Terumo Life Science Foundation (to M.M.).
PY - 2002/4/1
Y1 - 2002/4/1
N2 - Using the redox-active DNA conjugate (ferrocene-modified oligonucleotide 12 mer) as a probe, the novel electrochemical gene sensor, which is sensitive, convenient, and not relying on radio isotope, has been developed. Oligonucleotide (16 met) complementary to the target (19 met) was immobilized onto gold electrode through the specific chemisorption of successive phosphorothioates which were introduced into 5′-end of the oligonucleotide. The sequence of the conjugate was designed to be also complementary to another site of the target. Therefore, the conjugate and the oligonucleotide anchoring on the electrode formed a sandwich-type ternary complex with a target DNA to give electric currents based on the ferrocene oxidation. By using this system, we have distinguished the mutant that has one base substitution from the fully complementary target.
AB - Using the redox-active DNA conjugate (ferrocene-modified oligonucleotide 12 mer) as a probe, the novel electrochemical gene sensor, which is sensitive, convenient, and not relying on radio isotope, has been developed. Oligonucleotide (16 met) complementary to the target (19 met) was immobilized onto gold electrode through the specific chemisorption of successive phosphorothioates which were introduced into 5′-end of the oligonucleotide. The sequence of the conjugate was designed to be also complementary to another site of the target. Therefore, the conjugate and the oligonucleotide anchoring on the electrode formed a sandwich-type ternary complex with a target DNA to give electric currents based on the ferrocene oxidation. By using this system, we have distinguished the mutant that has one base substitution from the fully complementary target.
UR - http://www.scopus.com/inward/record.url?scp=0036532355&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036532355&partnerID=8YFLogxK
U2 - 10.1016/S0039-9140(01)00659-2
DO - 10.1016/S0039-9140(01)00659-2
M3 - Article
AN - SCOPUS:0036532355
SN - 0039-9140
VL - 56
SP - 857
EP - 866
JO - Talanta
JF - Talanta
IS - 5
ER -