TY - JOUR
T1 - DNA content of the B chromosomes in grasshopper Podisma kanoi Storozh. (Orthoptera, Acrididae)
AU - Bugrov, Alexander G.
AU - Karamysheva, Tatiana V.
AU - Perepelov, Eugeny A.
AU - Elisaphenko, Eugeny A.
AU - Rubtsov, Denis N.
AU - Warchałowska-Śliwa, Elzbieta
AU - Tatsuta, Haruki
AU - Rubtsov, Nikolay B.
N1 - Funding Information:
The authors thank Dr Shin-ichi Akimoto and Dr Masahiro Ohara, for all-round help in Japan. Part of this study was realized in the Hokkaido University Museum by A. Bugrov as Visiting Professor in 2005. The study was supported by a grant from the Presidium of the Russian Academy of Sciences FBiodiversity and genome dynamics_ for A. Bugrov; the Russian Fund of Basic Researches (RFBR) No. 05-04-48230a for T. Karamysheva; 05-04-48221a and 06-04-08211 for N. Rubtsov; a grant by the Polish State Committee for Scientific Reasearch (KBN) no. 2P04C 08826 for E. Warchałowska-S´liwa and A. Bugrov; and Grant-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan (no. 16770069 to H. Tatsuta). Thanks are also due to the Ministry of Environment of Japan for providing approval to collect materials in Joeshin-etsu National Park, Honshu, Japan.
PY - 2007/4
Y1 - 2007/4
N2 - A DNA library derived from the B chromosome of Podisma kanoi was obtained by chromosome microdissection. A total of 153 DNA clones were isolated from the microdissected DNA library. Twenty of them were sequenced. A comparison of B chromosome DNA sequences with sequences of other species from the DDBJ/GenBank/EMBL database ( http://www.ddbj.nig.ac.jp/ ) was performed. Different patterns of signals were observed after FISH with labeled cloned DNA fragments. FISH signals with cloned DNA fragments painted either whole Bs or their different regions. Some clones also gave signals in pericentromeric regions of A chromosomes. Other cloned DNA fragments gave only background-like signals on A and B chromosomes. Comparative FISH analysis of B chromosomes in Podisma kanoi and P. sapporensis with DNA probes derived from the Bs of these species revealed homologous DNA that was confined within pericentromeric and telometric regions of the B chromosome in P. kanoi. In contrast to the B chromosomes in P. sapporensis containing large regions enriched with rDNA, only a small cluster of rDNA was detected in one of the examined B chromosomes in P. kanoi. The data strongly suggest an independent origin of B chromosomes in two closely related Podisma species.
AB - A DNA library derived from the B chromosome of Podisma kanoi was obtained by chromosome microdissection. A total of 153 DNA clones were isolated from the microdissected DNA library. Twenty of them were sequenced. A comparison of B chromosome DNA sequences with sequences of other species from the DDBJ/GenBank/EMBL database ( http://www.ddbj.nig.ac.jp/ ) was performed. Different patterns of signals were observed after FISH with labeled cloned DNA fragments. FISH signals with cloned DNA fragments painted either whole Bs or their different regions. Some clones also gave signals in pericentromeric regions of A chromosomes. Other cloned DNA fragments gave only background-like signals on A and B chromosomes. Comparative FISH analysis of B chromosomes in Podisma kanoi and P. sapporensis with DNA probes derived from the Bs of these species revealed homologous DNA that was confined within pericentromeric and telometric regions of the B chromosome in P. kanoi. In contrast to the B chromosomes in P. sapporensis containing large regions enriched with rDNA, only a small cluster of rDNA was detected in one of the examined B chromosomes in P. kanoi. The data strongly suggest an independent origin of B chromosomes in two closely related Podisma species.
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U2 - 10.1007/s10577-007-1128-z
DO - 10.1007/s10577-007-1128-z
M3 - Article
C2 - 17406993
AN - SCOPUS:34248165013
SN - 0967-3849
VL - 15
SP - 315
EP - 326
JO - Chromosome Research
JF - Chromosome Research
IS - 3
ER -