TY - JOUR
T1 - Differential expression of sarcoplasmic and myofibrillar proteins of rat soleus muscle during denervation atrophy
AU - Sato, Yusuke
AU - Shimizu, Motoyuki
AU - Mizunoya, Wataru
AU - Wariishi, Hiroyuki
AU - Tatsumi, Ryuichi
AU - Buchman, Vladimir L.
AU - Ikeuchi, Yoshihide
N1 - Funding Information:
We wish to thank Dr. Neuberger, Medical Research Council Laboratory of Molecular Biology, Cambridge, for valuable suggestions regarding APOBEC-2. This work was partly supported by ITO Memorial Foundation, Tokyo, Japan to YI and the Wellcome Trust Grand to VLB.
PY - 2009
Y1 - 2009
N2 - Denervation is known to induce skeletal muscle atrophy and fiber-type transitions, the molecular mechanisms of which are poorly understood. To investigate the effect of denervation on skeletal muscle, proteomic analysis was performed to compare denervated soleus muscle with normal soleus muscle. The muscles were fractionated to myofibrillar and sarcoplasmic fractions, which were analysed using two-dimensional gel electro-phoresis (2-DE), followed by MALDI-TOF-MS. At least 30 differentially regulated proteins were identified in the sarcoplasmic fractions of normal and denervated soleus muscles. This group included metabolic enzymes, signaling molecules, chaperones, and contractile proteins. We also found two proteins, APOBEC-2 (RNA-editing enzyme) and Gamma-synuclein (breast cancer related protein), which have not been recognized as denervation-induced proteins to date. Our results might prove to be beneficial in elucidating the molecular mechanisms of denervation-induced muscle atrophy.
AB - Denervation is known to induce skeletal muscle atrophy and fiber-type transitions, the molecular mechanisms of which are poorly understood. To investigate the effect of denervation on skeletal muscle, proteomic analysis was performed to compare denervated soleus muscle with normal soleus muscle. The muscles were fractionated to myofibrillar and sarcoplasmic fractions, which were analysed using two-dimensional gel electro-phoresis (2-DE), followed by MALDI-TOF-MS. At least 30 differentially regulated proteins were identified in the sarcoplasmic fractions of normal and denervated soleus muscles. This group included metabolic enzymes, signaling molecules, chaperones, and contractile proteins. We also found two proteins, APOBEC-2 (RNA-editing enzyme) and Gamma-synuclein (breast cancer related protein), which have not been recognized as denervation-induced proteins to date. Our results might prove to be beneficial in elucidating the molecular mechanisms of denervation-induced muscle atrophy.
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U2 - 10.1271/bbb.90085
DO - 10.1271/bbb.90085
M3 - Article
C2 - 19661702
AN - SCOPUS:69949156922
SN - 0916-8451
VL - 73
SP - 1748
EP - 1756
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 8
ER -
