TY - GEN
T1 - Dielectrophoretic characteristics of microbeads labeled with DNA of various lengths
AU - Ding, Zhenhao
AU - Kasahara, Hiromichi
AU - Nakano, Michihiko
AU - Suehiro, Junya
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2015
Y1 - 2015
N2 - Polymerase chain reaction (PCR) is one of the most sensitive and specific detection methods of bacterial and viral infections. The authors proposed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads. The method is based on dramatic alteration of DEP characteristics of microbeads caused by DNA labelling. DNA labeled microbeads are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. DEP-trapped microbeads can be measured impedimetrically to realize rapid and quantitative detection of the amplified DNA. In this study, it was aimed to reveal how DNA length affects DEP characteristic of DNA-labeled microbeads. Dielectrophoretic crossover from the negative to the positive was measured for microbeads labeled with DNA length in 204 bp, 391 bp and 796 bp. After theoretical fitting of DEP crossover data, it was revealed that the surface conductance increased when the length of labeled DNA increased.
AB - Polymerase chain reaction (PCR) is one of the most sensitive and specific detection methods of bacterial and viral infections. The authors proposed a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads. The method is based on dramatic alteration of DEP characteristics of microbeads caused by DNA labelling. DNA labeled microbeads are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. DEP-trapped microbeads can be measured impedimetrically to realize rapid and quantitative detection of the amplified DNA. In this study, it was aimed to reveal how DNA length affects DEP characteristic of DNA-labeled microbeads. Dielectrophoretic crossover from the negative to the positive was measured for microbeads labeled with DNA length in 204 bp, 391 bp and 796 bp. After theoretical fitting of DEP crossover data, it was revealed that the surface conductance increased when the length of labeled DNA increased.
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U2 - 10.5220/0005280701850189
DO - 10.5220/0005280701850189
M3 - Conference contribution
AN - SCOPUS:84936803083
T3 - BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015
SP - 185
EP - 189
BT - BIODEVICES 2015 - 8th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 8th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2015
A2 - Cliquet, Alberto
A2 - Cliquet, Alberto
A2 - Fred, Ana
A2 - Gamboa, Hugo
A2 - Elias, Dirk
PB - SciTePress
T2 - 8th International Conference on Biomedical Electronics and Devices, BIODEVICES 2015
Y2 - 12 January 2015 through 15 January 2015
ER -