Dexamethasone and nitric oxide synthase gene expression in brain

Eiji Suzuki, Futoshi Shintani, Toshio Nakaki, Kiyoshi Nagata, Yasushi Yamazoe, Nobuchika Fujita, Masahiro Asai, Shigenobu Kanba

研究成果: ジャーナルへの寄稿学術誌査読

10 被引用数 (Scopus)


Systemic administration of lipopolysaccharide (LPS), which causes endotoxemia and systemic inflammation, has been reported to induce expression of the gene for type II inducible nitric oxide synthase (iNOS) in peripheral organs. This study was carried out to examine whether intraperitoneally injected LPS elicits the expression of iNOS messenger ribonucleic acid (mRNA) in the rat brain. We also investigated whether intraperitoneal treatment with dexamethasone (DEX) prevents this induction. To determine levels of iNOS mRNA, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method was employed. Treatment with LPS induced the expression of iNOS mRNA in various brain regions, accounting for approximately 1 x 105 to 4 x 105 molecules per μg of poly A+ RNA, and these inductions were markedly suppressed by DEX. The results suggest that, during systemic inflammation, iNOS mRNA induction occurs in brain through a DEX-sensitive mechanism.

ジャーナルJournal of Psychiatry and Neuroscience
出版ステータス出版済み - 1997

!!!All Science Journal Classification (ASJC) codes

  • 精神医学および精神衛生
  • 生物学的精神医学
  • 薬理学(医学)


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