descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques

Kohei Otomo; Takaki Omura; Yuki Nozawa; Steven J. Edwards; Yukihiko Sato; Yuri Saito; Shigehiro Yagishita; Hitoshi Uchida; Yuki Watakabe; Kiyotada Naitou; Rin Yanai; Naruhiko Sahara; Satoshi Takagi; Ryohei Katayama; Yusuke Iwata; Toshiro Shiokawa; Yoku Hayakawa; Kensuke Otsuka; Haruko Watanabe-Takano; Yuka Haneda; Shigetomo Fukuhara; Miku Fujiwara; Takenobu Nii; Chikara Meno; Naoki Takeshita; Kenta Yashiro; Juan Marcelo Rosales Rocabado; Masaru Kaku; Tatsuya Yamada; Yumiko Oishi; Hiroyuki Koike; Yinglan Cheng; Keisuke Sekine; Jun Ichiro Koga; Kaori Sugiyama; Kenichi Kimura; Fuyuki Karube; Hyeree Kim; Ichiro Manabe; Tomomi Nemoto; Kazuki Tainaka; Akinobu Hamada; Hjalmar Brismar; Etsuo A. Susaki

doi:10.1038/s41467-024-49131-1

descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques

Kohei Otomo, Takaki Omura, Yuki Nozawa, Steven J. Edwards, Yukihiko Sato, Yuri Saito, Shigehiro Yagishita, Hitoshi Uchida, Yuki Watakabe, Kiyotada Naitou, Rin Yanai, Naruhiko Sahara, Satoshi Takagi, Ryohei Katayama, Yusuke Iwata, Toshiro Shiokawa, Yoku Hayakawa, Kensuke Otsuka, Haruko Watanabe-Takano, Yuka HanedaShigetomo Fukuhara, Miku Fujiwara, Takenobu Nii, Chikara Meno, Naoki Takeshita, Kenta Yashiro, Juan Marcelo Rosales Rocabado, Masaru Kaku, Tatsuya Yamada, Yumiko Oishi, Hiroyuki Koike, Yinglan Cheng, Keisuke Sekine, Jun Ichiro Koga, Kaori Sugiyama, Kenichi Kimura, Fuyuki Karube, Hyeree Kim, Ichiro Manabe, Tomomi Nemoto, Kazuki Tainaka, Akinobu Hamada, Hjalmar Brismar, Etsuo A. Susaki

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

6 被引用数 (Scopus)

抄録

Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000–50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced (https://github.com/dbsb-juntendo/descSPIM), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.

本文言語	英語
論文番号	4941
ジャーナル	Nature communications
巻	15
号	1
DOI	https://doi.org/10.1038/s41467-024-49131-1
出版ステータス	出版済み - 12月 2024
外部発表	はい

!!!All Science Journal Classification (ASJC) codes

化学一般
生化学、遺伝学、分子生物学一般
物理学および天文学一般

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.1038/s41467-024-49131-1

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Otomo, K., Omura, T., Nozawa, Y., Edwards, S. J., Sato, Y., Saito, Y., Yagishita, S., Uchida, H., Watakabe, Y., Naitou, K., Yanai, R., Sahara, N., Takagi, S., Katayama, R., Iwata, Y., Shiokawa, T., Hayakawa, Y., Otsuka, K., Watanabe-Takano, H., ... Susaki, E. A. (2024). descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques. Nature communications, 15(1), 記事 4941. https://doi.org/10.1038/s41467-024-49131-1

Otomo, K, Omura, T, Nozawa, Y, Edwards, SJ, Sato, Y, Saito, Y, Yagishita, S, Uchida, H, Watakabe, Y, Naitou, K, Yanai, R, Sahara, N, Takagi, S, Katayama, R, Iwata, Y, Shiokawa, T, Hayakawa, Y, Otsuka, K, Watanabe-Takano, H, Haneda, Y, Fukuhara, S, Fujiwara, M, Nii, T , Meno, C, Takeshita, N, Yashiro, K, Rosales Rocabado, JM, Kaku, M, Yamada, T, Oishi, Y, Koike, H, Cheng, Y, Sekine, K, Koga, JI, Sugiyama, K, Kimura, K, Karube, F, Kim, H, Manabe, I, Nemoto, T, Tainaka, K, Hamada, A, Brismar, H & Susaki, EA 2024, 'descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques', Nature communications, vol. 15, no. 1, 4941. https://doi.org/10.1038/s41467-024-49131-1

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abstract = "Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000–50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced (https://github.com/dbsb-juntendo/descSPIM), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.",

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AU - Yagishita, Shigehiro

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AU - Sahara, Naruhiko

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AU - Iwata, Yusuke

AU - Shiokawa, Toshiro

AU - Hayakawa, Yoku

AU - Otsuka, Kensuke

AU - Watanabe-Takano, Haruko

AU - Haneda, Yuka

AU - Fukuhara, Shigetomo

AU - Fujiwara, Miku

AU - Nii, Takenobu

AU - Meno, Chikara

AU - Takeshita, Naoki

AU - Yashiro, Kenta

AU - Rosales Rocabado, Juan Marcelo

AU - Kaku, Masaru

AU - Yamada, Tatsuya

AU - Oishi, Yumiko

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AU - Cheng, Yinglan

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AU - Koga, Jun Ichiro

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AU - Brismar, Hjalmar

AU - Susaki, Etsuo A.

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descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques

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!!!All Science Journal Classification (ASJC) codes

UN SDG

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