De novo DNA methylation drives 5hmC accumulation in mouse zygotes

Rachel Amouroux, Buhe Nashun, Kenjiro Shirane, Shoma Nakagawa, Peter W.S. Hill, Zelpha D'Souza, Manabu Nakayama, Masashi Matsuda, Aleksandra Turp, Elodie Ndjetehe, Vesela Encheva, Nobuaki R. Kudo, Haruhiko Koseki, Hiroyuki Sasaki, Petra Hajkova

    研究成果: ジャーナルへの寄稿学術誌査読

    180 被引用数 (Scopus)

    抄録

    Zygotic epigenetic reprogramming entails genome-wide DNA demethylation that is accompanied by Tet methylcytosine dioxygenase 3 (Tet3)-driven oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC; refs 1-4). Here we demonstrate using detailed immunofluorescence analysis and ultrasensitive LC-MS-based quantitative measurements that the initial loss of paternal 5mC does not require 5hmC formation. Small-molecule inhibition of Tet3 activity, as well as genetic ablation, impedes 5hmC accumulation in zygotes without affecting the early loss of paternal 5mC. Instead, 5hmC accumulation is dependent on the activity of zygotic Dnmt3a and Dnmt1, documenting a role for Tet3-driven hydroxylation in targeting de novo methylation activities present in the early embryo. Our data thus provide further insights into the dynamics of zygotic reprogramming, revealing an intricate interplay between DNA demethylation, de novo methylation and Tet3-driven hydroxylation.

    本文言語英語
    ページ(範囲)225-233
    ページ数9
    ジャーナルNature Cell Biology
    18
    2
    DOI
    出版ステータス出版済み - 1月 28 2016

    !!!All Science Journal Classification (ASJC) codes

    • 細胞生物学

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