TY - GEN
T1 - Cytoplasmic transfer between live single cells by achieving transient cytoplasmic connection
AU - Wada, Ken Ichi
AU - Hosokawa, Kazuo
AU - Ito, Yoshihiro
AU - Maeda, Mizuo
N1 - Funding Information:
KAKENHI grant number 16K07207,
Funding Information:
This work was partly supported by JSPS Program, and RIKEN Single Cell Project.
Funding Information:
This work was partly supported by JSPS KAKENHI grant number 16K07207, JST Center of Innovation Program, and RIKEN Single Cell Project.
Publisher Copyright:
© 17CBMS-0001.
PY - 2020
Y1 - 2020
N2 - We previously developed a microfluidic device which can promote strictured cytoplasmic connection between live single cells by inducing cell fusion through a microslit. The promotion of cytoplasmic connection led to cytoplasmic transfer to the fusion partner; however, many of the fused cells did not recover to two independent cells. In this study, we developed a novel microfluidic device in which microslit was replaced with microtunnel. Cell fusion through a microtunnel resulted in promotion of transient cytoplasmic connection and peroxisome transfer to the fusion partner. The results demonstrate a feasible method for cytoplasmic transfer between live single cells.
AB - We previously developed a microfluidic device which can promote strictured cytoplasmic connection between live single cells by inducing cell fusion through a microslit. The promotion of cytoplasmic connection led to cytoplasmic transfer to the fusion partner; however, many of the fused cells did not recover to two independent cells. In this study, we developed a novel microfluidic device in which microslit was replaced with microtunnel. Cell fusion through a microtunnel resulted in promotion of transient cytoplasmic connection and peroxisome transfer to the fusion partner. The results demonstrate a feasible method for cytoplasmic transfer between live single cells.
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M3 - Conference contribution
AN - SCOPUS:85079595778
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 928
EP - 929
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -