Cytochrome c3 Modified Artificial Liposome. Structure, Electron Transport, and pH Gradient Generation

Iwao Tabushi, Takako Nishiya, Masatsugu Shimomuraf, Toyoki Kunitake, Hiroo Inokuchi, Tatsuhiko Yagi

研究成果: ジャーナルへの寄稿学術誌査読

34 被引用数 (Scopus)

抄録

Detailed studies on the structure, electron transport rates, and proton transport rates coupled to electron transport were carried out for artificial lecithin liposomes functionalized with cyt c3 or cyt c. Artificial liposomes modified with cyt c3 were found to be single-walled, normal liposomes of ca. 250-Å diameter based on electron micrographs. The electron transport rate across the membrane was very much accelerated in the presence of cyt c3, and the electron influx was coupled with rapid proton transport from the outside to the inside of the artificial liposome, generating a large pH gradient across the membrane. The maximum pH gradient is determined by the accelerated proton influx and slower passive proton efflux. The permeability coefficients of passive proton flow, PH+, and of accelerated coupled proton flow, PH+, were estimated. The observed permeability for the coupled transport is by a factor of l01-l02 larger than the usual passive proton permeability.

本文言語英語
ページ(範囲)219-226
ページ数8
ジャーナルJournal of the American Chemical Society
106
1
DOI
出版ステータス出版済み - 1月 1984
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 触媒
  • 化学一般
  • 生化学
  • コロイド化学および表面化学

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