TY - JOUR
T1 - CSF cytokine and chemokine profiles in acute disseminated encephalomyelitis
AU - Ishizu, Takaaki
AU - Minohara, Motozumi
AU - Ichiyama, Takashi
AU - Kira, Ryutaro
AU - Tanaka, Masahito
AU - Osoegawa, Manabu
AU - Hara, Toshiro
AU - Furukawa, Susumu
AU - Kira, Jun ichi
N1 - Funding Information:
This study was supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan, the Neuroimmunological Disease Research Committee and the Ministry of Health, Labor and Welfare of Japan for Research on Brain Science.
PY - 2006/6
Y1 - 2006/6
N2 - We simultaneously measured 16 cytokines/chemokines in cerebrospinal fluid (CSF) from 14 patients with acute disseminated encephalomyelitis (ADEM) and 20 controls using a fluorescent bead-based immunoassay. A variety of cytokines, such as IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IFN-γ, TNF-α, G-CSF and MIP-1β, were significantly elevated in ADEM. In particular, G-CSF showed a marked 38-fold increase compared to the control mean. Significant positive correlations with inflammatory parameters in CSF, such as cell counts and protein levels, were found for IFN-γ, IL-6 and IL-8. In contrast, IL-17 produced by activated CD4+ memory T cells was not increased. The results suggested that various cytokines related to activation of macrophages/microglias and Th1 and Th2 cells are upregulated in CSF in ADEM.
AB - We simultaneously measured 16 cytokines/chemokines in cerebrospinal fluid (CSF) from 14 patients with acute disseminated encephalomyelitis (ADEM) and 20 controls using a fluorescent bead-based immunoassay. A variety of cytokines, such as IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IFN-γ, TNF-α, G-CSF and MIP-1β, were significantly elevated in ADEM. In particular, G-CSF showed a marked 38-fold increase compared to the control mean. Significant positive correlations with inflammatory parameters in CSF, such as cell counts and protein levels, were found for IFN-γ, IL-6 and IL-8. In contrast, IL-17 produced by activated CD4+ memory T cells was not increased. The results suggested that various cytokines related to activation of macrophages/microglias and Th1 and Th2 cells are upregulated in CSF in ADEM.
KW - Acute disseminated encephalomyelitis
KW - Cerebrospinal fluid
KW - Chemokine
KW - Cytokine
UR - https://www.scopus.com/pages/publications/33646760288
UR - https://www.scopus.com/pages/publications/33646760288#tab=citedBy
U2 - 10.1016/j.jneuroim.2006.03.020
DO - 10.1016/j.jneuroim.2006.03.020
M3 - Article
C2 - 16697050
AN - SCOPUS:33646760288
SN - 0165-5728
VL - 175
SP - 52
EP - 58
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -