TY - JOUR
T1 - Crystal structure of the overlapping dinucleosome composed of hexasome and octasome
AU - Kato, Daiki
AU - Osakabe, Akihisa
AU - Arimura, Yasuhiro
AU - Mizukami, Yuka
AU - Horikoshi, Naoki
AU - Saikusa, Kazumi
AU - Akashi, Satoko
AU - Nishimura, Yoshifumi
AU - Park, Sam Yong
AU - Nogami, Jumpei
AU - Maehara, Kazumitsu
AU - Ohkawa, Yasuyuki
AU - Matsumoto, Atsushi
AU - Kono, Hidetoshi
AU - Inoue, Rintaro
AU - Sugiyama, Masaaki
AU - Kurumizaka, Hitoshi
N1 - Funding Information:
This work was supported in part by JSPS KAKENHI grant nos. JP25116002 (to H.Ku.), JP25116010 (to Y.O.), JP25116003 (to H.Ko.), JP15K18515 (to R.I.), JP15H02042 and JP16H01306 (to M.S.), and CREST, JST (to H.Ku. and Y.O.). This research is partially supported by the Platform Project for Supporting Drug Discovery and Life Science Research (Platform for Drug Discovery, Informatics, and Structural Life Science) from Japan Agency for Medical Research and Development (AMED) (to H.Ku., H.Ko., S.A., and Y.N.). H.Ku. and N.H. were supported by the Waseda Research Institute for Science and Engineering. D.K. was supported by a Research Fellowship from JSPS for Young Scientists (16J06850). This work was also supported by the Project for Construction of the Basis for the Advanced Materials Science and Analytical Study by the Innovative Use of Quantum Beam and Nuclear Sciences in the Research Reactor Institute of Kyoto University (to M.S and R.I.).
PY - 2017/4/14
Y1 - 2017/4/14
N2 - Nucleosomes are dynamic entities that are repositioned along DNA by chromatin remodeling processes. A nucleosome repositioned by the switch-sucrose nonfermentable (SWI/SNF) remodeler collides with a neighbor and forms the intermediate "overlapping dinucleosome." Here, we report the crystal structure of the overlapping dinucleosome, in which two nucleosomes are associated, at 3.14-angstrom resolution. In the overlapping dinucleosome structure, the unusual "hexasome" nucleosome, composed of the histone hexamer lacking one H2A-H2B dimer from the conventional histone octamer, contacts the canonical "octasome" nucleosome, and they intimately associate. Consequently, about 250 base pairs of DNA are left-handedly wrapped in three turns, without a linker DNA segment between the hexasome and octasome moieties. The overlapping dinucleosome structure may provide important information to understand how nucleosome repositioning occurs during the chromatin remodeling process.
AB - Nucleosomes are dynamic entities that are repositioned along DNA by chromatin remodeling processes. A nucleosome repositioned by the switch-sucrose nonfermentable (SWI/SNF) remodeler collides with a neighbor and forms the intermediate "overlapping dinucleosome." Here, we report the crystal structure of the overlapping dinucleosome, in which two nucleosomes are associated, at 3.14-angstrom resolution. In the overlapping dinucleosome structure, the unusual "hexasome" nucleosome, composed of the histone hexamer lacking one H2A-H2B dimer from the conventional histone octamer, contacts the canonical "octasome" nucleosome, and they intimately associate. Consequently, about 250 base pairs of DNA are left-handedly wrapped in three turns, without a linker DNA segment between the hexasome and octasome moieties. The overlapping dinucleosome structure may provide important information to understand how nucleosome repositioning occurs during the chromatin remodeling process.
UR - http://www.scopus.com/inward/record.url?scp=85018498469&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018498469&partnerID=8YFLogxK
U2 - 10.1126/science.aak9867
DO - 10.1126/science.aak9867
M3 - Article
C2 - 28408607
AN - SCOPUS:85018498469
SN - 0036-8075
VL - 356
SP - 205
EP - 208
JO - Science
JF - Science
IS - 6334
M1 - 356
ER -