CpG DNA/zymosan complex to enhance cytokine secretion owing to the cocktail effect

Takahisa Anada, Naoko Okada, Jusaku Minari, Ryouji Karinaga, Masami Mizu, Kazuya Koumoto, Seiji Shinkai, Kazuo Sakurai

研究成果: ジャーナルへの寄稿学術誌査読

11 被引用数 (Scopus)


Zymosan, classified among β-(1→3)-d-glucans, is produced from the cell wall of yeast and well known to induce proinflammatory cytokines when ingested by immune cells. We found that zymosan forms a complex with immunostimulatory CpG DNA, where both zymosan and CpG DNA can induce cytokine secretion according to the different mechanisms (i.e., recognized by different receptors). The complex activated macrophages and induced cytokine secretion, more efficiently than separate administration of zymosan or CpG DNA. Microscopic observation showed that this increment of the cytokine secretion can be explained by the fact that zymosan and zymosan/CpG DNA complex are up-taken more than naked CpG DNA. Additionally, existence of two different immunostimulants in the same cells may enhance the immunoresponse. This report presents a new strategy to construct a delivering vehicle for CpG DNA and to enhance its activity with the 'cocktail effect' of the two immunostimulants.

ジャーナルBioorganic and Medicinal Chemistry Letters
出版ステータス出版済み - 3月 1 2006

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学


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