CPA induces a sustained increase in [Ca2+](i) of endothelial cells in situ and relaxes porcine coronary artery

Yoshihiro Higuchi, Junji Nishimura, Sei Kobayashi, Hideo Kanaide

研究成果: ジャーナルへの寄稿学術誌査読

22 被引用数 (Scopus)

抄録

Using fura 2 fluorometry, we investigated the effect of cyclopiazonic acid (CPA), an inhibitor of Ca2+ pump adenosinetriphosphatase of the endoplasmic reticulum, on cytosolic Ca2+ concentration ([Ca2+ ](i)) and tension in porcine aortic valvular endothelial cells and coronary arterial strips with endothelium. In normal physiological salt solution, CPA induced a sustained increase in [Ca2+](i) in the valvular strips, whereas in Ca2+- free physiological salt solution, CPA elicited a transient elevation of [Ca2+](i). CPA (30 μM) relaxed coronary strips with endothelium precontracted by 100 nM U-46619; this relaxation was partially inhibited by N(ω)-nitro-L-arginine (100 μM). These results indicate that the CPA- induced increase in [Ca2+](i) depends on the Ca2+ release and the Ca2+ influx in the endothelial cells in situ and that the CPA-induced endothelium- dependent decreases in [Ca2+](i) and tension in the smooth muscle are due to the combined effect of N(ω)-nitro-L-arginine-sensitive and -resistant factors.

本文言語英語
ページ(範囲)H2038-H2049
ジャーナルAmerican Journal of Physiology - Heart and Circulatory Physiology
270
6 39-6
DOI
出版ステータス出版済み - 6月 1996
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生理学
  • 循環器および心血管医学
  • 生理学(医学)

フィンガープリント

「CPA induces a sustained increase in [Ca2+](i) of endothelial cells in situ and relaxes porcine coronary artery」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル