Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody

Masataka Suzuki, Masashige Shinkai, Hiroyuki Honda, Masamichi Kamihira, Shinji Iijima, Takeshi Kobayashi

研究成果: ジャーナルへの寄稿学術誌査読

6 被引用数 (Scopus)

抄録

Cells expressing a membrane-anchored single-chain fragment variable (scFv) domain against a tumor-specific antibody were fabricated. These cells were able to bind to cells of a human colon cancer line (BM314) expressing the erbB-2 proto-oncogene. A plasmid, pMFverbB, was first constructed in which the anti-ErbB-2 scFv gene was cloned in-frame between a signal peptide sequence and the platelet-derived growth factor receptor (PDGFR) transmembrane domain gene to express scFv on the cell surface. An African green monkey cell line, COS-1, was stably transfected with pMFverbB. Immunofluorescence assay experiments and microscopic observation showed that the cells expressing scFv bound to the human tumor cells overexpressing the ErbB-2 protein as well as to cells of a mouse fibroblast line (NIH-3T3) transfected with the erbB-2 gene. The cells expressing scFv could take up magnetite cationic liposomes as a model of particle-type drug and retained the ability to target ErbB-2-expressing cells. The fabricated cells have the potential to serve as drug carriers in drug targeting applications.

本文言語英語
ページ(範囲)191-196
ページ数6
ジャーナルBiochimica et Biophysica Acta - General Subjects
1525
1-2
DOI
出版ステータス出版済み - 2月 16 2001
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学

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