Connexin43 plays an important role in lung development

Kouji Nagata, Kouji Masumoto, Genshirou Esumi, Risa Teshiba, Keigo Yoshizaki, Satoshi Fukumoto, Kazuaki Nonaka, Tomoaki Taguchi

研究成果: ジャーナルへの寄稿学術誌査読

17 被引用数 (Scopus)


Objectives: Connexin43 (Cx43) is one of the proteins associated with gap junction. Connexin43 knockout mice die after birth owing to hypoplastic lungs. The purpose of this study was to analyze the hypoplastic lung of Cx43 knockout mice to clarify the role of the Cx43 during lung development. Methods: Adult hetero Cx43 mice were mated. Newborn mice were divided into the following groups: wild, hetero, and knockout. Total RNA was extracted from the right lung, and the left lung was fixed for immunohistochemical staining. The mRNA expression of surfactant protein C, aquaporin-5, and α-smooth muscle actin were analyzed by reverse transcriptase polymerase chain reaction. H&E and immunohistochemical staining for those markers were performed. Results: The mRNA expression of aquaporin-5, surfactant protein C, and α-smooth muscle actin was significantly lower in knockout mice than that in the wild and hetero mice. H&E staining in the knockout mice showed narrow airspaces and thicker interalveolar septae. Immunohistochemical staining in all markers showed the formation of alveoli to be delayed in the knockout mice. Conclusion: Based on these findings, Cx43 is closely related to alveolar and vascular formation during lung development.

ジャーナルJournal of Pediatric Surgery
出版ステータス出版済み - 12月 2009

!!!All Science Journal Classification (ASJC) codes

  • 外科
  • 小児科学、周産期医学および子どもの健康


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