TY - JOUR
T1 - Comparison of amino terminal region of three isoenzymes of phospholipases A2 (TFV PL-Ia, TFV PL-Ib, TFV PL-X) from Trimeresurus flavoviridis (habu snake) venom and the complete amino acid sequence of the basic phospholipase, TFV PL-X
AU - Kini, R. Manjunatha
AU - Kawabata, Shun Ichiro
AU - Iwanaga, Sadaaki
N1 - Funding Information:
Acknowledgements - Theauthors thank Dr T. Swro, National Institute ofHealth, Toltyo, for the generous gift of baba snake venom and Dr T. Mtvwrw of Kyushu Uaiveraity for critical suggestions during the work. We appreciate Mrs C. SueYOSxt's help in amino acid analyses . One of the authors (RMK) thanks the Japanese Government for financial assistance .
PY - 1986
Y1 - 1986
N2 - R. M. Kini, S.-I. Kawabata and S. Iwanaga. Comparison of amino terminal region of three isoenzymes of phospholipases A2 (TFV OL-Ia, TFV PL-Ib, TFV PL-X) from Trimeresurus flavoviridis (habu snake) venom and the complete amino acid sequence of the basic phospholipase, TFV PL-X. Toxicon 24, 1117 - 1129, 1986.-Three phospholipases (PLA2s) isolated from Japanese habu snake (Trimeresurus flavoviridis) venom differ from each other in the physiological symptoms induced in animals. The amino acid compositions and amino terminal sequences of these isoenzymes have been compared. TFV PL-Ia and TFV PL-Ib resemble each other very closely, but TFV PL-X has a distinctly different amino acid composition and amino terminal region. The complete amino acid sequence of the basic PLA2, TFV PL-X, was determined by sequencing the four peptides obtained by the cleavage of Asp - Pro bonds. The overlapping peptide was obtained by cleaving tryptophanyl bond and the carboxy terminal region was determined by sequencing the chymotryptic peptide. TFV PL-X consisted of a single chain of 122 amino acid residues, including 14 half-cystine residues. It is a group II A PLA2 and is homologous with the phospholipase super family. However, it has a distinctly different sequence compared with the other PLA2s from the venom of Trimeresurus species.
AB - R. M. Kini, S.-I. Kawabata and S. Iwanaga. Comparison of amino terminal region of three isoenzymes of phospholipases A2 (TFV OL-Ia, TFV PL-Ib, TFV PL-X) from Trimeresurus flavoviridis (habu snake) venom and the complete amino acid sequence of the basic phospholipase, TFV PL-X. Toxicon 24, 1117 - 1129, 1986.-Three phospholipases (PLA2s) isolated from Japanese habu snake (Trimeresurus flavoviridis) venom differ from each other in the physiological symptoms induced in animals. The amino acid compositions and amino terminal sequences of these isoenzymes have been compared. TFV PL-Ia and TFV PL-Ib resemble each other very closely, but TFV PL-X has a distinctly different amino acid composition and amino terminal region. The complete amino acid sequence of the basic PLA2, TFV PL-X, was determined by sequencing the four peptides obtained by the cleavage of Asp - Pro bonds. The overlapping peptide was obtained by cleaving tryptophanyl bond and the carboxy terminal region was determined by sequencing the chymotryptic peptide. TFV PL-X consisted of a single chain of 122 amino acid residues, including 14 half-cystine residues. It is a group II A PLA2 and is homologous with the phospholipase super family. However, it has a distinctly different sequence compared with the other PLA2s from the venom of Trimeresurus species.
UR - https://www.scopus.com/pages/publications/0022967150
UR - https://www.scopus.com/pages/publications/0022967150#tab=citedBy
U2 - 10.1016/0041-0101(86)90138-8
DO - 10.1016/0041-0101(86)90138-8
M3 - Article
C2 - 3564060
AN - SCOPUS:0022967150
SN - 0041-0101
VL - 24
SP - 1117
EP - 1129
JO - Toxicon
JF - Toxicon
IS - 11-12
ER -