TY - JOUR
T1 - Cloning and expression of two carbonyl reductase-like 20β-hydroxysteroid dehydrogenase cDNAs in ovarian follicles of rainbow trout (Oncorhynchus mykiss)
AU - Guan, Guijun
AU - Tanaka, Minoru
AU - Todo, Takashi
AU - Young, Graham
AU - Yoshikuni, Michiyasu
AU - Nagahama, Yoshitaka
N1 - Funding Information:
We thank the staff of the Samegai Trout Hatchery for providing trout and laboratory facilities. These studies were supported in part by Grants-in-Aids for Research for the Future (JSPS-RFTF 96L100401), Priority Areas (07283104) and Grants-in Aids (08454266, 10440247). Support from the Japanese Ministry of Edu- cation and from the University of Otago Research Committee to GY is gratefully acknowledged.
PY - 1999/2/5
Y1 - 1999/2/5
N2 - In salmonid fish, 20β-hydroxysteroid dehydrogenase (20β-HSD) is a key enzyme involved in the production of oocyte maturation-inducing hormone (MIH), 17α,20β-dihydroxy-4-pregnen-3-one. Here we report the isolation of two cDNAs which encode proteins with high homology to carbonyl reductase-like 20β-HSD (CR/20β-HSD) from rainbow trout (Oncorhynchus mykiss) ovarian follicles. Genomic DNA analysis showed that the two CR/20β-HSD cDNAs are derived from two different genes. Northern blot and RT PCR analysis demonstrated that trout CR/20β-HSDs are broadly expressed in various tissues. Enzymatic characterization using recombinant CR/20β-HSD proteins produced in E. coli showed that the product of one of the two cDNAs had both 20β-HSD and CR activity, but the other had neither activity. Although the functional significance of the two genes remains unresolved, these results clearly demonstrate the presence of two distinct CR/20β-HSD transcripts in the trout ovary.
AB - In salmonid fish, 20β-hydroxysteroid dehydrogenase (20β-HSD) is a key enzyme involved in the production of oocyte maturation-inducing hormone (MIH), 17α,20β-dihydroxy-4-pregnen-3-one. Here we report the isolation of two cDNAs which encode proteins with high homology to carbonyl reductase-like 20β-HSD (CR/20β-HSD) from rainbow trout (Oncorhynchus mykiss) ovarian follicles. Genomic DNA analysis showed that the two CR/20β-HSD cDNAs are derived from two different genes. Northern blot and RT PCR analysis demonstrated that trout CR/20β-HSDs are broadly expressed in various tissues. Enzymatic characterization using recombinant CR/20β-HSD proteins produced in E. coli showed that the product of one of the two cDNAs had both 20β-HSD and CR activity, but the other had neither activity. Although the functional significance of the two genes remains unresolved, these results clearly demonstrate the presence of two distinct CR/20β-HSD transcripts in the trout ovary.
UR - https://www.scopus.com/pages/publications/0033525160
UR - https://www.scopus.com/pages/publications/0033525160#tab=citedBy
U2 - 10.1006/bbrc.1998.0127
DO - 10.1006/bbrc.1998.0127
M3 - Article
C2 - 10082666
AN - SCOPUS:0033525160
SN - 0006-291X
VL - 255
SP - 123
EP - 128
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -