Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2

Masayasu Hayashi; Kazumitsu Maehara; Akihito Harada; Yuichiro Semba; Kensuke Kudo; Hidehisa Takahashi; Shinya Oki; Chikara Meno; Kenji Ichiyanagi; Koichi Akashi; Yasuyuki Ohkawa

doi:10.1002/jcb.25368

Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2

Masayasu Hayashi, Kazumitsu Maehara, Akihito Harada, Yuichiro Semba, Kensuke Kudo, Hidehisa Takahashi, Shinya Oki, Chikara Meno, Kenji Ichiyanagi, Koichi Akashi, Yasuyuki Ohkawa

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

28 被引用数 (Scopus)

抄録

Chd5 is an essential factor for neuronal differentiation and spermatogenesis and is a known tumor suppressor. H3K27me3 and H3K4un are modifications recognized by Chd5; however, it remains unclear how Chd5 remodels chromatin structure. We completely disrupted the Chd5 locus using the CRISPR-Cas9 system to generate a 52 kbp long deletion and analyzed Chd5 function in mouse embryonic stem cells. Our findings show that Chd5 represses murine endogenous retrovirus-L (MuERV-L/MERVL), an endogenous retrovirus-derived retrotransposon, by regulating H3K27me3 and H3.1/H3.2 function. J. Cell. Biochem. 117: 780-792, 2016.

本文言語	英語
ページ（範囲）	780-792
ページ数	13
ジャーナル	Journal of Cellular Biochemistry
巻	117
号	3
DOI	https://doi.org/10.1002/jcb.25368
出版ステータス	出版済み - 3月 2016

!!!All Science Journal Classification (ASJC) codes

生化学
分子生物学
細胞生物学

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title = "Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2",

abstract = "Chd5 is an essential factor for neuronal differentiation and spermatogenesis and is a known tumor suppressor. H3K27me3 and H3K4un are modifications recognized by Chd5; however, it remains unclear how Chd5 remodels chromatin structure. We completely disrupted the Chd5 locus using the CRISPR-Cas9 system to generate a 52 kbp long deletion and analyzed Chd5 function in mouse embryonic stem cells. Our findings show that Chd5 represses murine endogenous retrovirus-L (MuERV-L/MERVL), an endogenous retrovirus-derived retrotransposon, by regulating H3K27me3 and H3.1/H3.2 function. J. Cell. Biochem. 117: 780-792, 2016.",

keywords = "CHROMATIN REMODELING ENZYME, Chd5, RETROTRANSPOSON, STEM CELL BIOLOGY",

author = "Masayasu Hayashi and Kazumitsu Maehara and Akihito Harada and Yuichiro Semba and Kensuke Kudo and Hidehisa Takahashi and Shinya Oki and Chikara Meno and Kenji Ichiyanagi and Koichi Akashi and Yasuyuki Ohkawa",

note = "Publisher Copyright: {\textcopyright} 2015 Wiley Periodicals, Inc.",

year = "2016",

month = mar,

doi = "10.1002/jcb.25368",

language = "English",

volume = "117",

pages = "780--792",

journal = "Journal of Cellular Biochemistry",

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TY - JOUR

T1 - Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2

AU - Hayashi, Masayasu

AU - Maehara, Kazumitsu

AU - Harada, Akihito

AU - Semba, Yuichiro

AU - Kudo, Kensuke

AU - Takahashi, Hidehisa

AU - Oki, Shinya

AU - Meno, Chikara

AU - Ichiyanagi, Kenji

AU - Akashi, Koichi

AU - Ohkawa, Yasuyuki

PY - 2016/3

Y1 - 2016/3

N2 - Chd5 is an essential factor for neuronal differentiation and spermatogenesis and is a known tumor suppressor. H3K27me3 and H3K4un are modifications recognized by Chd5; however, it remains unclear how Chd5 remodels chromatin structure. We completely disrupted the Chd5 locus using the CRISPR-Cas9 system to generate a 52 kbp long deletion and analyzed Chd5 function in mouse embryonic stem cells. Our findings show that Chd5 represses murine endogenous retrovirus-L (MuERV-L/MERVL), an endogenous retrovirus-derived retrotransposon, by regulating H3K27me3 and H3.1/H3.2 function. J. Cell. Biochem. 117: 780-792, 2016.

AB - Chd5 is an essential factor for neuronal differentiation and spermatogenesis and is a known tumor suppressor. H3K27me3 and H3K4un are modifications recognized by Chd5; however, it remains unclear how Chd5 remodels chromatin structure. We completely disrupted the Chd5 locus using the CRISPR-Cas9 system to generate a 52 kbp long deletion and analyzed Chd5 function in mouse embryonic stem cells. Our findings show that Chd5 represses murine endogenous retrovirus-L (MuERV-L/MERVL), an endogenous retrovirus-derived retrotransposon, by regulating H3K27me3 and H3.1/H3.2 function. J. Cell. Biochem. 117: 780-792, 2016.

KW - CHROMATIN REMODELING ENZYME

KW - Chd5

KW - RETROTRANSPOSON

KW - STEM CELL BIOLOGY

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U2 - 10.1002/jcb.25368

DO - 10.1002/jcb.25368

M3 - Article

C2 - 26359639

AN - SCOPUS:84955501754

SN - 0730-2312

VL - 117

SP - 780

EP - 792

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

IS - 3

ER -

Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2

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!!!All Science Journal Classification (ASJC) codes

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