Characterization of B16 melanoma-specific cytotoxic T lymphocytes

Mamoru Harada, Koji Tamada, Koichiro Abe, Tieli Li, Yasuhiro Onoe, Hitoshi Tada, Katsunori Tatsugami, Takashi Ando, Genki Kimura, Kikuo Nomoto

研究成果: ジャーナルへの寄稿学術誌査読

17 被引用数 (Scopus)

抄録

A B16 melanoma-specific CD8+ T cell line (AB1) was established from the spleen cells of C57BL/6 mice cured of B16 melanoma with interleukin (IL)-12 treatment. The ABl line exclusively used T cell receptor V(β11). The AB1 cells exhibited a cytolytic activity against both syngeneic B16 melanoma and allogeneic P815 mastocytoma, whereas a cold inhibition assay revealed specificity of the AB1 cells against B16 melanoma. Their lostability to kill a class I loss variant of B16 melanoma was restored by the transfection of H- 2K(b) gene. In addition, their interferon (IFN)-γ production was significantly suppressed by the addition of anti-H-2K(b) monoclonal antibody, and RT-PCR analysis showed that the AB1 line expressed the mRNA encoding IFN- γ, but not IL-4 or IL-10. The experiment using synthetic peptides of tyrosinase-related protein-2 (TRP-2) revealed that the AB1 cells could recognize TRP-2181-188 peptide. Moreover, the AB1 cells showed an in vivo antitumor effect against established pulmonary metastases of B16 melanoma. Overall, these results indicate that the Tcl-type V(β11) + AB1 cells exert an antitumor activity against syngeneic B16 melanoma through recognition of TRP-2181-188 peptide in an H-2K(b)-restricted manner.

本文言語英語
ページ(範囲)198-204
ページ数7
ジャーナルCancer Immunology Immunotherapy
47
4
DOI
出版ステータス出版済み - 1998
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学
  • 腫瘍学
  • 癌研究

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