TY - JOUR
T1 - Characterization of a novel superoxide dismutase in Nilaparvata lugens
AU - Yamamoto, Kohji
AU - Yamaguchi, Misuzu
N1 - Funding Information:
We are grateful to Dr. Tetsuro Shinoda at the National Institute of Agrobiological Sciences (Tsukuba, Japan) for useful discussions on N. lugens.
Publisher Copyright:
© 2021 Wiley Periodicals LLC
PY - 2022/2
Y1 - 2022/2
N2 - The brown planthopper (Nilaparvata lugens) is a major agricultural pest of rice crops. Analysis of the enzymes produced by N. lugens is important to develop pest-control methods. Superoxide dismutase (SOD) is a detoxification enzyme that catalyzes the conversion of superoxide anions (reactive oxygen species) into oxygen and hydrogen peroxide. As there have been no reports on SOD in N. lugens, in this study, we characterized a new SOD in the brown planthopper, nlSOD1. Amino acid sequence and phylogenetic analyses revealed that nlSOD1 is a member of the Cu/Zn-SOD family. Recombinant nlSOD1, when overexpressed in Escherichia coli, catalyzes the dismutation of superoxide radicals into molecular O2 and H2O2. Exposure to various insecticides induced nlSOD1 messenger RNA expression. These results indicate that nlSOD1 may contribute to the insecticide resistance of N. lugens. The findings of this study may assist in the development of novel methods to control the population of N. lugens.
AB - The brown planthopper (Nilaparvata lugens) is a major agricultural pest of rice crops. Analysis of the enzymes produced by N. lugens is important to develop pest-control methods. Superoxide dismutase (SOD) is a detoxification enzyme that catalyzes the conversion of superoxide anions (reactive oxygen species) into oxygen and hydrogen peroxide. As there have been no reports on SOD in N. lugens, in this study, we characterized a new SOD in the brown planthopper, nlSOD1. Amino acid sequence and phylogenetic analyses revealed that nlSOD1 is a member of the Cu/Zn-SOD family. Recombinant nlSOD1, when overexpressed in Escherichia coli, catalyzes the dismutation of superoxide radicals into molecular O2 and H2O2. Exposure to various insecticides induced nlSOD1 messenger RNA expression. These results indicate that nlSOD1 may contribute to the insecticide resistance of N. lugens. The findings of this study may assist in the development of novel methods to control the population of N. lugens.
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U2 - 10.1002/arch.21862
DO - 10.1002/arch.21862
M3 - Article
C2 - 34897778
AN - SCOPUS:85121027157
SN - 0739-4462
VL - 109
JO - Archives of insect biochemistry and physiology
JF - Archives of insect biochemistry and physiology
IS - 2
M1 - e21862
ER -