Celecoxib inhibits osteoblast maturation by suppressing the expression of Wnt target genes

Akihiro Nagano, Masaki Arioka, Fumi Takahashi-Yanaga, Etsuko Matsuzaki, Toshiyuki Sasaguri

研究成果: ジャーナルへの寄稿学術誌査読

29 被引用数 (Scopus)

抄録

Non-steroidal anti-inflammatory drugs (NSAIDs) have been shown to impair bone healing. We previously reported that in colon cancer cells, celecoxib, a COX-2-selective NSAID, inhibited the canonical Wnt/β-catenin signaling pathway. Since this pathway also plays an important role in osteoblast growth and differentiation, we examined the effect of celecoxib on maturation of osteoblast-like cell line MC3T3-E1. Celecoxib induced degradation of transcription factor 7-like 2, a key transcription factor of the canonical Wnt pathway. Subsequently, we analyzed the effect of celecoxib on two osteoblast differentiation markers; runt-related transcription factor 2 (RUNX2) and alkaline phosphatase (ALP), both of which are the products of the canonical Wnt pathway target genes. Celecoxib inhibited the expression of both RUNX2 and ALP by suppressing their promoter activity. Consistent with these observations, celecoxib also strongly inhibited osteoblast-mediated mineralization. These results suggest that celecoxib inhibits osteoblast maturation by suppressing Wnt target genes, and this could be the mechanism that NSAIDs inhibit bone formation and fracture healing.

本文言語英語
ページ(範囲)18-24
ページ数7
ジャーナルJournal of Pharmacological Sciences
133
1
DOI
出版ステータス出版済み - 1月 1 2017

!!!All Science Journal Classification (ASJC) codes

  • 分子医療
  • 薬理学

フィンガープリント

「Celecoxib inhibits osteoblast maturation by suppressing the expression of Wnt target genes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル