CD90-(Thy-1-) high selection enhances reprogramming capacity of murine adipose-derived mesenchymal stem cells

Koichi Kawamoto, Masamitsu Konno, Hiroaki Nagano, Shimpei Nishikawa, Yoshito Tomimaru, Hirofumi Akita, Naoki Hama, Hiroshi Wada, Shogo Kobayashi, Hidetoshi Eguchi, Masahiro Tanemura, Toshinori Ito, Yuichiro Doki, Masaki Mori, Hideshi Ishii

研究成果: ジャーナルへの寄稿学術誌査読

14 被引用数 (Scopus)


Background. Mesenchymal stem cells (MSCs), including adipose tissue-derived mesenchymal stem cells (ADSC), are multipotent and can differentiate into various cell types possessing unique immunomodulatory features. Several clinical trials have demonstrated the safety and possible efficacy of MSCs in organ transplantation.Thus, stem cell therapy is promising for tolerance induction. In this study, we assessed the reprogramming capacity of murine ADSCs and found that CD90 (Thy-1), originally discovered as a thymocyte antigen, could be a useful marker for cell therapy. Method. Murine ADSCs were isolated from B6 mice, sorted using a FACSAria cell sorter by selection of CD90Hi or CD90Lo, and then transduced with four standard factors (4F; Oct4, Sox2, Klf4, and c-Myc). Results. Unsorted,CD90Hi-sorted, and CD90Lo-sortedmurine ADSCs were reprogrammed using standard 4F transduction. CD90Hi ADSCs showed increased numbers of alkaline phosphatase-positive colonies compared with CD90Lo ADSCs. The relative reprogramming efficiencies of unsorted, CD90Hi-sorted, and CD90Lo-sorted ADSCs were 100%, 116.5%, and 74.7%, respectively. CD90Hi cells weremore responsive to reprogramming. Conclusion. CD90Hi ADSCs had greater reprogramming capacity than CD90 Lo ADSCs, suggesting that ADSCs have heterogeneous subpopulations. Thus, CD90Hi selection presents an effective strategy to isolate a highly suppressive subpopulation for stem cell-based tolerance induction therapy.

ジャーナルDisease Markers
出版ステータス出版済み - 2013

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学
  • 臨床生化学
  • 生化学、医学


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