TY - JOUR
T1 - BUBR1 insufficiency is correlated with eNOS reduction experimentally in vitro and in vivo, and in gastric cancer tissue
AU - Kawakubo, Eisuke
AU - Matsumoto, Takuya
AU - Yoshiya, Keiji
AU - Yamashita, Sho
AU - Jogo, Tomoko
AU - Saeki, Hiroshi
AU - Oki, Eiji
AU - Furuyama, Tadashi
AU - Oda, Yoshinao
AU - Maehara, Yoshihiko
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science (Grant No. 25462164 to TM) and Uehara Memorial Foundation.
Publisher Copyright:
© 2018 International Institute of Anticancer Research.All right reserved.
PY - 2018/11
Y1 - 2018/11
N2 - Background/Aim: Budding uninhibited by benzimidazole-related 1 (BUBR1) and endothelial nitric oxide synthase (eNOS) are related to aging and angiogenesis. This study examined the effect of low BUBR1 expression on eNOS expression in vivo, in vitro, and human gastric cancer tissues. Materials and Methods: Human umbilical vein endothelial cells (HUVECs) were passaged to investigate the effect of aging on BUBR1 and eNOS expression; expression of eNOS and phospho-eNOS protein was assessed in BUBR1 siRNAtransfected HUVECs. Additionally, guanosine 3′,5′ cyclic monophosphate (cGMP) and eNOS protein levels were measured in BUBR1-insufficient mice (Bubr1L/-). BUBR1 and eNOS expression levels were also evaluated in human gastric cancer tissues. Results: BUBR1 and eNOS, but not p-eNOS, levels were reduced significantly in aged and BUBR1 siRNAtransfected HUVECs. Additionally, cGMP production and the eNOS protein level were reduced in Bubr1L/- mice. Human gastric cancer tissues with low BUBR1 expression showed no eNOS expression. Conclusion: A decrease in BUBR1 reduced eNOS bioavailability through a pathway other than eNOS phosphorylation.
AB - Background/Aim: Budding uninhibited by benzimidazole-related 1 (BUBR1) and endothelial nitric oxide synthase (eNOS) are related to aging and angiogenesis. This study examined the effect of low BUBR1 expression on eNOS expression in vivo, in vitro, and human gastric cancer tissues. Materials and Methods: Human umbilical vein endothelial cells (HUVECs) were passaged to investigate the effect of aging on BUBR1 and eNOS expression; expression of eNOS and phospho-eNOS protein was assessed in BUBR1 siRNAtransfected HUVECs. Additionally, guanosine 3′,5′ cyclic monophosphate (cGMP) and eNOS protein levels were measured in BUBR1-insufficient mice (Bubr1L/-). BUBR1 and eNOS expression levels were also evaluated in human gastric cancer tissues. Results: BUBR1 and eNOS, but not p-eNOS, levels were reduced significantly in aged and BUBR1 siRNAtransfected HUVECs. Additionally, cGMP production and the eNOS protein level were reduced in Bubr1L/- mice. Human gastric cancer tissues with low BUBR1 expression showed no eNOS expression. Conclusion: A decrease in BUBR1 reduced eNOS bioavailability through a pathway other than eNOS phosphorylation.
UR - http://www.scopus.com/inward/record.url?scp=85056115651&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056115651&partnerID=8YFLogxK
U2 - 10.21873/anticanres.12960
DO - 10.21873/anticanres.12960
M3 - Article
C2 - 30396924
AN - SCOPUS:85056115651
SN - 0250-7005
VL - 38
SP - 6099
EP - 6106
JO - Anticancer research
JF - Anticancer research
IS - 11
ER -
