Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags

Richi Sakaguchi, Marcus N. Leiwe, Takeshi Imai

研究成果: ジャーナルへの寄稿学術誌査読

44 被引用数 (Scopus)

抄録

The stochastic multicolor labeling method ‘Brainbow’ is a powerful strategy to label multiple neurons differentially with fluorescent proteins; however, the fluorescence levels provided by the original attempts to use this strategy were inadequate. In the present study, we developed a stochastic multicolor labeling method with enhanced expression levels that uses a tetracycline-operator system (Tetbow). We optimized Tetbow for either plasmid or virus vector-mediated multicolor labeling. When combined with tissue clearing, Tetbow was powerful enough to visualize the three-dimensional architecture of individual neurons. Using Tetbow, we were able to visualize the axonal projection patterns of individual mitral/tufted cells along several millimeters in the mouse olfactory system. We also developed a Tetbow system with chemical tags, in which genetically encoded chemical tags were labeled with synthetic fluorophores. This was useful in expanding the repertoire of the fluorescence labels and the applications of the Tetbow system. Together, these new tools facilitate light-microscopy-based neuronal tracing at both a large scale and a high resolution.

本文言語英語
論文番号e40350
ジャーナルeLife
7
DOI
出版ステータス出版済み - 11月 1 2018

!!!All Science Journal Classification (ASJC) codes

  • 免疫学および微生物学一般
  • 生化学、遺伝学、分子生物学一般
  • 神経科学一般

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