TY - JOUR
T1 - Blood Oxygen Sensor Using a Boron-Doped Diamond Electrode
AU - Triana, Yunita
AU - Ogata, Genki
AU - Tomisaki, Mai
AU - Irkham,
AU - Einaga, Yasuaki
N1 - Funding Information:
We thank Professor Motoaki Sano (School of Medicine, Keio University) for useful discussions. The following research grants partially supported this work: Grant-in-Aid for Scientific Research B 21H03805 (to G.O.), and Grant-in-Aid for Scientific Research A 19H00832 (to Y.E.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. In addition, Y.T. was supported by the Indonesia Endowment Fund for Education Scholarship from the Ministry of Finance, LPDP.
Publisher Copyright:
© 2022 American Chemical Society.
PY - 2022/3/8
Y1 - 2022/3/8
N2 - The electrochemical behavior of oxygen (O2) in blood was studied using boron-doped diamond (BDD) electrodes. Cyclic voltammogram of O2 in a 0.1 M phosphate buffer solution solution containing 1 × 10-6 M of bovine hemoglobin exhibits a reduction peak at -1.4 V (vs Ag/AgCl). Moreover, the scan rate dependence was investigated to study the reduction reaction mechanism, which was attributable to the reduction of O2 to H2O2 via two electrons. A linear calibration curve was observed in the concentration range of 86.88-314.63 mg L-1 (R2 = 0.99) with a detection limit of 1.0 mg L-1 (S/B = 3). The analytical performance was better than those with glassy carbon or platinum electrodes as the working electrode. In addition, an application to bovine blood was performed. The O2 concentration in the blood measured on the BDD electrodes was compared to that measured using an OxyLite Pro fiber-optic oxygen sensor device. Both methods gave similar values of the O2 concentration in the range of ∼40 to 150 mmHg. This result confirms that BDD electrodes could potentially be used to detect the O2 concentration in blood.
AB - The electrochemical behavior of oxygen (O2) in blood was studied using boron-doped diamond (BDD) electrodes. Cyclic voltammogram of O2 in a 0.1 M phosphate buffer solution solution containing 1 × 10-6 M of bovine hemoglobin exhibits a reduction peak at -1.4 V (vs Ag/AgCl). Moreover, the scan rate dependence was investigated to study the reduction reaction mechanism, which was attributable to the reduction of O2 to H2O2 via two electrons. A linear calibration curve was observed in the concentration range of 86.88-314.63 mg L-1 (R2 = 0.99) with a detection limit of 1.0 mg L-1 (S/B = 3). The analytical performance was better than those with glassy carbon or platinum electrodes as the working electrode. In addition, an application to bovine blood was performed. The O2 concentration in the blood measured on the BDD electrodes was compared to that measured using an OxyLite Pro fiber-optic oxygen sensor device. Both methods gave similar values of the O2 concentration in the range of ∼40 to 150 mmHg. This result confirms that BDD electrodes could potentially be used to detect the O2 concentration in blood.
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U2 - 10.1021/acs.analchem.1c04999
DO - 10.1021/acs.analchem.1c04999
M3 - Article
AN - SCOPUS:85125646417
SN - 0003-2700
VL - 94
SP - 3948
EP - 3955
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 9
ER -