Bacteria species that are present in the aqueous phase in the separation of water from crude oil in Amarume oil well in Japan are mainly pseudomonas species, gammaproteobacteria classis and shewanella species. The water including mixed bacteria was agitated with contaminated soil. The petroleum hydrocarbon in the wasted clay could be decomposed to 70% for one month after the treatment of alkali and hydrogen peroxide agitation. Also synthetic dioxins in artificial mud could be decomposed 80% and in artificial soil could be done 40% over one month. These bacteria showed very effective bioremediation of soil contaminated by oil and dioxins in laboratory.