Bessel-beam illumination Raman microscopy

Kazuki Bando; Shumpei Yabuuchi; Menglu Li; Toshiki Kubo; Ryosuke Oketani; Nicholas I. Smith; Katsumasa Fujita

doi:10.1364/BOE.456138

Bessel-beam illumination Raman microscopy

Kazuki Bando, Shumpei Yabuuchi, Menglu Li, Toshiki Kubo, Ryosuke Oketani, Nicholas I. Smith, Katsumasa Fujita

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

4 被引用数 (Scopus)

抄録

We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.

本文言語	英語
ページ（範囲）	3161-3170
ページ数	10
ジャーナル	Biomedical Optics Express
巻	13
号	6
DOI	https://doi.org/10.1364/BOE.456138
出版ステータス	出版済み - 6月 1 2022
外部発表	はい

!!!All Science Journal Classification (ASJC) codes

バイオテクノロジー
原子分子物理学および光学

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title = "Bessel-beam illumination Raman microscopy",

abstract = "We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.",

author = "Kazuki Bando and Shumpei Yabuuchi and Menglu Li and Toshiki Kubo and Ryosuke Oketani and Smith, {Nicholas I.} and Katsumasa Fujita",

note = "Funding Information: Acknowledgments. The authors thank Ryosuke Morisaki, Tomoyuki Murashima, and Takuma Shibata for advice on sensitivity evaluation and optical setup construction. This work was partially supported by JST-Mirai Program and JST-CREST program under Grant Numbers JPMJMI20G3 and JPMJCR1925, Japan. Publisher Copyright: {\textcopyright} 2022 Optica Publishing Group.",

year = "2022",

month = jun,

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doi = "10.1364/BOE.456138",

language = "English",

volume = "13",

pages = "3161--3170",

journal = "Biomedical Optics Express",

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TY - JOUR

T1 - Bessel-beam illumination Raman microscopy

AU - Bando, Kazuki

AU - Yabuuchi, Shumpei

AU - Li, Menglu

AU - Kubo, Toshiki

AU - Oketani, Ryosuke

AU - Smith, Nicholas I.

AU - Fujita, Katsumasa

N1 - Funding Information: Acknowledgments. The authors thank Ryosuke Morisaki, Tomoyuki Murashima, and Takuma Shibata for advice on sensitivity evaluation and optical setup construction. This work was partially supported by JST-Mirai Program and JST-CREST program under Grant Numbers JPMJMI20G3 and JPMJCR1925, Japan. Publisher Copyright: © 2022 Optica Publishing Group.

PY - 2022/6/1

Y1 - 2022/6/1

N2 - We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.

AB - We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.

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DO - 10.1364/BOE.456138

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JO - Biomedical Optics Express

JF - Biomedical Optics Express

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Bessel-beam illumination Raman microscopy

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!!!All Science Journal Classification (ASJC) codes

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