TY - JOUR
T1 - AVID mouse
T2 - A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice
AU - Ohnishi, Yuichiro
AU - Setoyama, Daiki
AU - Miwa, Hideki
AU - Koitabashi, Norimichi
AU - Ogasawara, Riki
AU - Kitada, Seri
AU - Matoba, Naoki
AU - Ayano, Takahito
AU - Hiramoto, Kaoru
AU - Yasui, Ryuto
AU - Sugiura, Yuki
AU - Anada, Takahisa
AU - Ino, Kosuke
AU - Matsuda, Hinako
AU - Noma, Takahiro
AU - Nonaka, Shigenori
AU - Izumi, Takashi
AU - Kurabayashi, Masahiko
AU - Suematsu, Makoto
AU - Kunisaki, Yuya
AU - Yanagita, Motoko
AU - Imamura, Hiromi
AU - Yamamoto, Masamichi
N1 - Publisher Copyright:
© 2025 The Author(s)
PY - 2025/9/23
Y1 - 2025/9/23
N2 - We developed the AVID (ATP visualization in vivo directly) mouse, a genetically encoded biosensor mouse enabling real-time, multiscale imaging of ATP dynamics across the whole body, organs, and cellular compartments in living animals. AVID revealed previously undetectable localized ATP depletion near the central vein of the liver after myocardial infarction, spatially linked to kynurenic acid accumulation—a phenomenon invisible to conventional bulk metabolomics. By seamlessly integrating macroscopic organ-level imaging with microscopic spatial metabolomics, AVID establishes a new framework for spatial systems metabolism. Beyond myocardial infarction, this platform offers broad applicability to study organ-organ metabolic communication, spatial metabolic heterogeneity, and localized metabolic shifts across diverse physiological and pathological contexts, providing a transformative resource for metabolic research.
AB - We developed the AVID (ATP visualization in vivo directly) mouse, a genetically encoded biosensor mouse enabling real-time, multiscale imaging of ATP dynamics across the whole body, organs, and cellular compartments in living animals. AVID revealed previously undetectable localized ATP depletion near the central vein of the liver after myocardial infarction, spatially linked to kynurenic acid accumulation—a phenomenon invisible to conventional bulk metabolomics. By seamlessly integrating macroscopic organ-level imaging with microscopic spatial metabolomics, AVID establishes a new framework for spatial systems metabolism. Beyond myocardial infarction, this platform offers broad applicability to study organ-organ metabolic communication, spatial metabolic heterogeneity, and localized metabolic shifts across diverse physiological and pathological contexts, providing a transformative resource for metabolic research.
KW - ATP dynamics
KW - CP: Metabolism
KW - FRET
KW - GO-ATeam biosensor
KW - disease progression
KW - energy metabolism
KW - in vivo imaging
KW - multiscale imaging
KW - myocardial infarction
KW - organ-organ interaction
KW - spatial systems metabolism
UR - https://www.scopus.com/pages/publications/105015554795
UR - https://www.scopus.com/pages/publications/105015554795#tab=citedBy
U2 - 10.1016/j.celrep.2025.116246
DO - 10.1016/j.celrep.2025.116246
M3 - Article
C2 - 40944910
AN - SCOPUS:105015554795
SN - 2639-1856
VL - 44
JO - Cell Reports
JF - Cell Reports
IS - 9
M1 - 116246
ER -