Automated acquisition of vast numbers of electron holograms with atomic-scale phase information

Yoshio Takahashi, Tetsuya Akashi, Atsuko Sato, Toshiaki Tanigaki, Hiroyuki Shinada, Yasukazu Murakami

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)


An automated acquisition system for collecting a large number of electron holograms, to improve the statistical precision of phase analysis, was developed. A technique for shifting the electron beam in combination with stage movement allows data to be acquired over a wide area of a TEM-specimen grid. Undesired drift in the hologram position, which may occur during the hologram acquisition, can be corrected in real time by automated detection of the interferencefringe region in an image. To demonstrate the usefulness of the developed automated hologram acquisition system, gold nanoparticles dispersed on a carbon foil were observed with a 1.2-MV atomic resolution holography electron microscope. The system could obtain 1024 holograms, which provided phase maps for more than 500 nanoparticles with a lateral resolution of 0.14 nm, in just 1 h. The observation results revealed an anomalous increase in mean inner potential for a particle size smaller than 4 nm. The developed automated hologram acquisition system can be applied to improve the precision of phase measurement by averaging many phase images, as demonstrated by single particle analysis for biological entities. Moreover, the system makes it possible to study electrostatic potential of catalysts and other functional nanoparticles at atomic resolution.

出版ステータス出版済み - 4月 1 2020

!!!All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 器械工学
  • 放射線学、核医学およびイメージング


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