Applicability of the DPPH assay for evaluating the antioxidant capacity of food additives - inter-laboratory evaluation study

Tomoko Shimamura, Yoshihiro Sumikura, Takeshi Yamazaki, Atsuko Tada, Takehiro Kashiwagi, Hiroya Ishikawa, Toshiro Matsui, Naoki Sugimoto, Hiroshi Akiyama, Hiroyuki Ukeda

研究成果: ジャーナルへの寄稿学術誌査読

140 被引用数 (Scopus)

抄録

An inter-laboratory evaluation study was conducted in order to evaluate the antioxidant capacity of food additives by using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Four antioxidants used as existing food additives (i.e., tea extract, grape seed extract, enju extract, and d-α-tocopherol) and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were used as analytical samples, and 14 laboratories participated in this study. The repeatability relative standard deviation (RSDr) of the IC50 of Trolox, four antioxidants, and the Trolox equivalent antioxidant capacity (TEAC) were 1.8 - 2.2%, 2.2 - 2.9%, and 2.1 - 2.5%, respectively. Thus, the proposed DPPH assay showed good performance within the same laboratory. The reproducibility relative standard deviation (RSDR) of IC50 of Trolox, four antioxidants, and TEAC were 4.0 - 7.9%, 6.0 - 11%, and 3.7 - 9.3%, respectively. The RSDR/RSDr values of TEAC were lower than, or nearly equal to, those of IC50 of the four antioxidants, suggesting that the use of TEAC was effective for reducing the variance among the laboratories. These results showed that the proposed DPPH assay could be used as a standard method to evaluate the antioxidant capacity of food additives.

本文言語英語
ページ(範囲)717-721
ページ数5
ジャーナルanalytical sciences
30
7
DOI
出版ステータス出版済み - 2014

!!!All Science Journal Classification (ASJC) codes

  • 分析化学

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